Imaging of oxygen and hypoxia in cell and tissue samples

Cellular and Molecular Life Sciences : CMLS
Dmitri B Papkovsky, Ruslan I Dmitriev

Abstract

Molecular oxygen (O2) is a key player in cell mitochondrial function, redox balance and oxidative stress, normal tissue function and many common disease states. Various chemical, physical and biological methods have been proposed for measurement, real-time monitoring and imaging of O2 concentration, state of decreased O2 (hypoxia) and related parameters in cells and tissue. Here, we review the established and emerging optical microscopy techniques allowing to visualize O2 levels in cells and tissue samples, mostly under in vitro and ex vivo, but also under in vivo settings. Particular examples include fluorescent hypoxia stains, fluorescent protein reporter systems, phosphorescent probes and nanosensors of different types. These techniques allow high-resolution mapping of O2 gradients in live or post-mortem tissue, in 2D or 3D, qualitatively or quantitatively. They enable control and monitoring of oxygenation conditions and their correlation with other biomarkers of cell and tissue function. Comparison of these techniques and corresponding imaging setups, their analytical capabilities and typical applications are given.

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Citations

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Methods Mentioned

BETA
fluorescence microscopy
biosensor
biosensors
imaging techniques
fluorescence imaging
transfection
FRET
transgenic

Software Mentioned

PLIM
PicoQuant
LaVision
FLIM
μs

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