Imaging of Programmed Cell Death Ligand 1: Impact of Protein Concentration on Distribution of Anti-PD-L1 SPECT Agents in an Immunocompetent Murine Model of Melanoma

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Jessie R NedrowGeorge Sgouros

Abstract

Programmed cell death ligand 1 (PD-L1) is part of an immune checkpoint system that is essential for preventing autoimmunity and cancer. Recent approaches in immunotherapy that target immune checkpoints have shown great promise in a variety of cancers, including metastatic melanoma. The use of targeted molecular imaging would help identify patients who will best respond to anti-PD-L1 treatment while potentially providing key information to limit immune-related adverse effects. Recently, we developed an antibody-based PD-L1-targeted SPECT agent-111In-diethylenetriaminepentaacetic acid (DTPA)-anti-PD-L1-to identify PD-L1-positive tumors in vivo. To best use such PD-L1-targeted imaging agents, it is important, as a first step, to understand how the signal is affected by different parameters. Methods: We evaluated the impact of protein concentration on the distribution of 111In-DTPA-anti-PD-L1 in a murine model of aggressive melanoma. Results:111In-DTPA-anti-PD-L1 (dissociation constant, 0.6 ± 0.1 nM) demonstrated increased uptake in B16F10 tumors at protein concentrations equaling or exceeding 1 mg/kg at 24 h and 3 mg/kg at 72 h. At 24 h, the PD-L1-rich spleen and lungs demonstrated decreasing uptake with increasing protein concent...Continue Reading

References

Feb 22, 2002·European Journal of Immunology·LauraL CarterBeatriz M Carreno
Mar 20, 2010·Physics in Medicine and Biology·Woutjan BranderhorstFreek J Beekman
Jan 13, 2012·Current Opinion in Immunology·Suzanne L TopalianDrew M Pardoll
Mar 23, 2012·Nature Reviews. Cancer·Drew M Pardoll
Jun 5, 2012·The New England Journal of Medicine·Julie R BrahmerJon M Wigginton
Mar 23, 2013·Annual Review of Immunology·Kong Chen, Jay K Kolls
Jun 4, 2013·The New England Journal of Medicine·Jedd D WolchokMario Sznol
Oct 11, 2013·Frontiers in Immunology·Donald B Palmer
Apr 10, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Janis M TaubeRobert A Anders
Apr 12, 2014·International Journal of Molecular Imaging·Pavan P AdiseshaiahScott E McNeil
Nov 18, 2014·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Sukhmani K PaddaHeather A Wakelee
Feb 11, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Moon-Young KimDoo Hyun Chung
Apr 22, 2015·The New England Journal of Medicine·Michael A PostowF Stephen Hodi
Jun 2, 2015·The New England Journal of Medicine·James LarkinJedd D Wolchok
Sep 1, 2015·Seminars in Oncology·Evan J LipsonSuzanne L Topalian
Nov 11, 2015·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Jessie R NedrowCarolyn J Anderson
Jul 23, 2016·Theranostics·Michael HettichGabriele Niedermann

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Citations

Nov 1, 2017·European Journal of Nuclear Medicine and Molecular Imaging·Weijun WeiWeibo Cai
Nov 21, 2018·Radiology·Mizuki NishinoF Stephen Hodi
May 17, 2019·Journal of Clinical Medicine·Alberto SignoreAndor W J M Glaudemans
Feb 8, 2019·Oncotarget·Heather M Gibson, Nerissa T Viola
Nov 7, 2019·EJNMMI Radiopharmacy and Chemistry·Peter WierstraSandra Heskamp
Jul 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yuhan ZhangFeng Wang
Dec 12, 2018·Advanced Materials·Weizhi Wang, Zhiyuan Hu
Nov 20, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Lin QiuHongcheng Shi
Oct 7, 2020·Journal for Immunotherapy of Cancer·Elly L van der VeenMarjolijn N Lub-de Hooge
Oct 20, 2020·Advanced Therapeutics·Corrine Ying Xuan ChuaAlessandro Grattoni
Jan 19, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Danique GiesenMartin Pool
Oct 17, 2020·Cancer Management and Research·Weiqing WangXue Meng
Feb 21, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Ingrid Julienne Georgette BurvenichAndrew Mark Scott
Mar 13, 2021·Advanced Drug Delivery Reviews·Carina PeresHelena F Florindo
Jan 20, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Aaron T Mayer, Sanjiv S Gambhir
May 26, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Aaron T Mayer, Sanjiv S Gambhir
Jan 12, 2020·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Susanne LütjeRalph A Bundschuh
Aug 18, 2020·Nuclear Medicine and Biology·Angel CortezJessie R Nedrow
May 26, 2018·ACS Combinatorial Science·Max A KruzikiBenjamin J Hackel
Mar 16, 2019·Molecular Pharmaceutics·Haley L WisslerRongsheng E Wang
Sep 10, 2021·Frontiers in Oncology·David LeungWendy Hayes
Sep 23, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Anna M Wu, Neeta Pandit-Taskar

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