Imaging of respiratory network topology in living brainstem slices

Molecular and Cellular Neurosciences
N HarteltS L Mironov

Abstract

Topology of neuronal networks contributes to their functioning but the structure-function relationships are not yet understood. In order to reveal the spatial organisation of the respiratory network, we expressed enhanced green fluorescent proteins in neurons in brainstem slices containing the respiratory kernel (pre-Bötzinger complex). The expression was neuron specific due to use of adeno-associated viral vector driving transgene expression from synapsin 1 promoter. Both neuronal cell bodies and their dendrites were labelled with high efficacy. This labelling allowed for enhanced spatial resolution as compared to conventional calcium-sensitive dyes. Neurons occupied about 10% of tissue volume and formed an interconnected network. Using custom-developed software, we quantified the network structure that had a modular structure consisting of clusters having transverse (dorso-ventral) orientation. They contained in average seven neurons and connections between the cells in different clusters were less frequent. This novel in situ imaging technique is promising to gain new knowledge about the fine structure and function of neuronal networks in living slice preparations.

References

May 23, 1998·The Journal of Physiology·S L MironovD W Richter
Feb 13, 2001·Trends in Neurosciences·F M Benes, N Lange
Mar 22, 2001·Nature·S H Strogatz
Nov 17, 2001·The Journal of Physiology·M HallerD W Richter
Nov 14, 2002·Molecular Neurobiology·Markus U Ehrengruber
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christoph StosiekArthur Konnerth
Apr 29, 2005·Neuropharmacology·S L Mironov, K Langohr
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason N D KerrFritjof Helmchen
Nov 15, 2005·The Journal of Physiology·John J GreerKlaus Ballanyi
Dec 31, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·E OhB Kahng
Feb 24, 2006·Nature Reviews. Neuroscience·Jack L Feldman, Christopher A Del Negro
Jul 6, 2006·The European Journal of Neuroscience·Jeffrey T Potts, Julian F R Paton
Aug 18, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Danny Eytan, Shimon Marom
Nov 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Araya RuangkittisakulKlaus Ballanyi
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christopher J HoneyOlaf Sporns

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Citations

Sep 21, 2012·Journal of Neurophysiology·Michael S Carroll, Jan-Marino Ramirez
Mar 31, 2009·BMC Neuroscience·S L MironovS Kügler
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Claire GuerrierDavid Holcman
Jan 30, 2013·Respiratory Physiology & Neurobiology·Gregory D Funk, John J Greer
Apr 21, 2009·Bio Systems·Ginette Horcholle-Bossavit, Brigitte Quenet
Jun 10, 2009·Respiratory Physiology & Neurobiology·Araya RuangkittisakulKlaus Ballanyi
Aug 8, 2014·Frontiers in Physiology·Andrés Nieto-PosadasFernando Peña-Ortega
Mar 25, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Sergej Mironov
Sep 27, 2018·PloS One·Saju Balakrishnan, Sergej L Mironov
May 3, 2008·The Journal of Physiology·Christopher A Del Negro, John A Hayes
Nov 8, 2011·Journal of Neural Engineering·Christopher FietkiewiczChristopher G Wilson
Dec 15, 2015·Journal of Neurophysiology·Wiktor S PhillipsJens C Rekling
Jun 16, 2017·Journal of Neurophysiology·Kameron Decker HarrisEric Shea-Brown
Nov 6, 2018·Frontiers in Cellular Neuroscience·Saju Balakrishnan, Sergej L Mironov
Jan 6, 2015·The Journal of Physiology·Jack L Feldman, Kaiwen Kam
Jan 28, 2021·Journal of Neurophysiology·Josué de Jesús Juárez-VidalesFernando Peña-Ortega

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