Imaging of retina cellular and subcellular structures using ptychographic hard X-ray tomography.

Journal of Cell Science
Valerie PanneelsGebhard Schertler

Abstract

Ptychographic hard X-ray computed tomography (PXCT) is a recent method allowing imaging with quantitative electron-density contrast. Here, we imaged, at cryogenic temperature and without sectioning, cellular and subcellular structures of a chemically fixed and stained wild-type mouse retina, including axons and synapses, with complete isotropic 3D information over tens of microns. Comparison with tomograms of degenerative retina from a mouse model of retinitis pigmentosa illustrates the potential of this method for analyzing disease processes like neurodegeneration at sub-200 nm resolution. As a non-destructive imaging method, PXCT is very suitable for correlative imaging. Within the outer plexiform layer containing the photoreceptor synapses, we identified somatic synapses. We used a small region inside the X-ray-imaged sample for further high-resolution focused ion beam/scanning electron microscope tomography. The subcellular structures of synapses obtained with the X-ray technique matched the electron microscopy data, demonstrating that PXCT is a powerful scanning method for tissue volumes of more than 60 cells and sensitive enough for identification of regions as small as 200 nm, which remain available for further structura...Continue Reading

References

Mar 14, 1975·Science·D H Anderson, S K Fisher
May 1, 1990·Journal of the Optical Society of America. A, Optics and Image Science·J Malik, P Perona
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M I NaashW Baehr
Aug 12, 2000·Neuron·S HaverkampH Wässle
Nov 28, 2002·The Journal of Comparative Neurology·Karen MigdaleStan Schein
Sep 21, 2004·Nature Reviews. Neuroscience·Heinz Wässle
Aug 30, 2005·Journal of Structural Biology·Marin van Heel, Michael Schatz
Mar 8, 2006·The European Journal of Neuroscience·M SamardzijaC Grimm
Sep 13, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marijana SamardzijaChristian Grimm
Jan 26, 2007·Advances in Experimental Medicine and Biology·Xi-Qin Ding, Muna I Naash
Feb 10, 2009·Ultramicroscopy·Pierre ThibaultFranz Pfeiffer
Apr 28, 2009·Journal of Synchrotron Radiation·P KraftB Schmitt
Mar 1, 2009·Journal of Electron Spectroscopy and Related Phenomena·M R HowellsD Starodub
Sep 25, 2010·Nature·Martin DierolfFranz Pfeiffer
Apr 29, 2011·Journal of Synchrotron Radiation·Sergey GorelickChristian David
Jul 22, 2011·Journal of Visualized Experiments : JoVE·Graham KnottMarco Cantoni
Nov 24, 2011·Optics Express·Manuel Guizar-SicairosOliver Bunk
Aug 3, 2012·The Review of Scientific Instruments·M HollerO Bunk
Feb 2, 2013·PloS One·Severin Reinhard HeynenChristian Grimm
Aug 9, 2013·Nature·Moritz HelmstaedterWinfried Denk
Dec 25, 2013·Journal of Synchrotron Radiation·S ChenS Vogt
Jun 13, 2014·Optics Express·Xiaojing HuangYong S Chu
Sep 2, 2014·Journal of Synchrotron Radiation·Pierre ThibaultAndreas Menzel
Apr 10, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shuai LiPeter G Fuerst
Apr 16, 2015·Cell and Tissue Research·C Wichmann, T Moser
May 13, 2015·Scientific Reports·Martin KrenkelTim Salditt
Sep 4, 2015·FEBS Letters·Juan D GoutmanMaría Eugenia Gómez-Casati
Nov 23, 2016·ELife·Barbara PfisterSamuel C Zeeman
Dec 14, 2016·Optics Express·Esther H R TsaiManuel Guizar-Sicairos
May 23, 2017·Methods in Cell Biology·Matthia A KarremanYannick Schwab
Jul 26, 2017·Scientific Reports·S H ShahmoradianM Holler
Dec 3, 2017·The Review of Scientific Instruments·M HollerM Vitins
Feb 3, 2018·Scientific Reports·Peng Li, Andrew Maiden
May 3, 2018·The Review of Scientific Instruments·M HollerO Bunk
Jun 20, 2018·Proceedings of the National Academy of Sciences of the United States of America·Mareike TöpperwienTim Salditt
Jun 6, 2019·Optics Express·Michal OdstrčilMirko Holler
Jun 20, 2019·Scientific Reports·Fabrizia FogliaPaul F McMillan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.