Imaging of single mRNA molecules moving within a living cell nucleus

Biochemical and Biophysical Research Communications
Hisashi TadakumaTakashi Funatsu

Abstract

In eukaryotic cells, pre-mRNAs are transcribed in the nucleus, processed by 5' capping, 3'-polyadenylation, and splicing, and exported to the cytoplasm for translation. To examine the nuclear mRNA transport mechanism, intron-deficient mRNAs of truncated beta-globin and EGFP were synthesized, fluorescently labeled in vitro, and injected into the nucleus of living Xenopus A6 cells. The trajectories of single mRNA molecules in the nucleus were visualized using video-rate confocal microscopy. Approximately half the mRNAs moved by Brownian motion in the nucleoplasm, except the nucleoli, with an apparent diffusion coefficient of 0.2microm(2)/s, about 1/150 of that in water. The slow diffusion could not be explained by simple diffusion obeying the Stokes-Einstein equation, suggesting interactions of the mRNAs with nuclear components. The remaining mRNAs were stationary with an average residence time of about 30s, comparable to the time required for mRNA diffusion from the site of synthesis to nuclear pores.

References

Feb 1, 1994·Biophysical Journal·M J Saxton
Aug 1, 1995·Biophysical Journal·M J Saxton
Mar 1, 1996·Biophysical Journal·M J Saxton
Jul 14, 1997·The Journal of Cell Biology·O SeksekA S Verkman
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J C PolitzT Pederson
Apr 21, 1999·Current Biology : CB·J C PolitzR H Singer
Sep 14, 2001·Biochemical and Biophysical Research Communications·H TadakumaT Funatsu
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·T KuesU Kubitscheck
Apr 5, 2002·Nature·Tom Maniatis, Robin Reed
May 8, 2002·Nature Reviews. Molecular Cell Biology·Gideon DreyfussNaoyuki Kataoka
Aug 10, 2002·Science·Dmytro NykypanchukDavid A Hoagland
Jan 28, 2003·Current Biology : CB·Dahlene FuscoEdouard Bertrand
Sep 10, 2003·Molecular Biology of the Cell·Joan C Ritland PolitzThoru Pederson
Jun 19, 2004·Science·Yaron Shav-TalRobert H Singer
Jan 3, 2006·Biophysical Journal·Joël BeaudouinJan Ellenberg
Feb 18, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuaki TokunagaTokio Tani

❮ Previous
Next ❯

Citations

Nov 28, 2012·Nature Cell Biology·Timothy T WeilIlan Davis
Oct 9, 2012·IET Systems Biology·M R Roussel, T Tang
Mar 3, 2009·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takashi Funatsu
Jan 15, 2014·Chemical Reviews·Sethuramasundaram PitchiayaNils G Walter
Jan 12, 2016·Scientific Reports·Sato EidaTakashi Nakamura
Oct 20, 2009·Biochemical and Biophysical Research Communications·Mai YamagishiTakashi Funatsu
Feb 28, 2009·Experimental Cell Research·Mai YamagishiTakashi Funatsu
Apr 9, 2009·Biochemical and Biophysical Research Communications·Yo Ishihama, Takashi Funatsu
Dec 7, 2007·Experimental Cell Research·Yo IshihamaTakashi Funatsu
Mar 23, 2007·Seminars in Cell & Developmental Biology·Alexis J RodriguezJason B Dictenberg
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Carl R Urbinati, Roy M Long
Apr 13, 2013·Nucleus·Jonathan Sheinberger, Yaron Shav-Tal
Jan 4, 2017·ACS Nano·Daniel JasinskiPeixuan Guo
May 22, 2019·Journal of Biophotonics·Jialei Tang, Kyu Young Han

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
Yo Ishihama, Takashi Funatsu
Proceedings of the National Academy of Sciences of the United States of America
Diana Y VargasSanjay Tyagi
Proceedings of the National Academy of Sciences of the United States of America
Jan Peter SiebrasseUlrich Kubitscheck
© 2022 Meta ULC. All rights reserved