Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction

American Journal of Physiology. Heart and Circulatory Physiology
Nicholas A FlavahanSheila Flavahan

Abstract

Experiments were performed to determine whether remodeling of the actin cytoskeleton contributes to arteriolar constriction. Mouse tail arterioles were mounted on cannulae in a myograph and superfused with buffer solution. The alpha1-adrenergic agonist phenylephrine (0.1-1 micromol/l) caused constriction that was unaffected by cytochalasin D (300 nmol/l) or latrunculin A (100 nmol/l), inhibitors of actin polymerization. In contrast, each compound abolished the mechanosensitive constriction (myogenic response) evoked by elevation in transmural pressure (PTM; 10-60 or 90 mmHg). Arterioles were fixed, permeabilized, and stained with Alexa-568 phalloidin and Alexa-488 DNAse I to visualize F-actin and G-actin, respectively, using a Zeiss 510 laser scanning microscope. Elevation in PTM, but not phenylephrine (1 micromol/l), significantly increased the intensity of F-actin and significantly decreased the intensity of G-actin staining in arteriolar vascular smooth muscle cells (VSMCs). The increase in F-actin staining caused by an elevation in PTM was inhibited by cytochalasin D. In VSMCs at 10 mmHg, prominent F-actin staining was restricted to the cell periphery, whereas after elevation in PTM, transcytoplasmic F-actin fibers were loc...Continue Reading

References

Feb 15, 1989·Archives of Biochemistry and Biophysics·E Urbanik, B R Ware
Jan 1, 1995·Annual Review of Cell and Developmental Biology·D A Schafer, J A Cooper
Sep 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J V Small
Apr 1, 1996·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·S Y SaitoH Karaki
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·A J ManiotisD E Ingber
Jun 17, 1998·Stroke; a Journal of Cerebral Circulation·M J Cipolla, G Osol
Jan 15, 1999·Acta Physiologica Scandinavica·J V Small, M Gimona
Apr 30, 1999·Physiological Reviews·M J Davis, M A Hill
Oct 3, 1999·Microscopy Research and Technique·I SpectorM R Bubb
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M A ChotaniN A Flavahan
Apr 12, 2000·The European Respiratory Journal·S J Gunst, D D Tang
Jun 5, 2001·Nature Cell Biology·R LittlefieldV M Fowler
Jul 21, 2001·Circulation Research·P T NowickiN A Flavahan
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Natalia I Gokina, George Osol
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Konstantin G BirukovJoe G N Garcia
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Barbara TolloczkoJames G Martin
Mar 6, 2003·Cell Calcium·O V PovstyanT B Bolton
Mar 29, 2003·Physiological Reviews·C G dos RemediosN J Nosworthy
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Alexander D BershadskyBenjamin Geiger
Oct 24, 2003·Trends in Cell Biology·Robert S Fischer, Velia M Fowler
Nov 1, 2003·The International Journal of Biochemistry & Cell Biology·Su Hao Lo
Feb 24, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luis A Martinez-LemusGerald A Meininger

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Citations

Jul 26, 2011·Medical & Biological Engineering & Computing·Samantha M Steelman, Jay D Humphrey
Jul 4, 2008·American Journal of Physiology. Cell Physiology·Hak Rim KimKathleen G Morgan
Jul 4, 2008·American Journal of Physiology. Cell Physiology·Susan J Gunst, Wenwu Zhang
Dec 21, 2007·Proceedings of the American Thoracic Society·Wenwu Zhang, Susan J Gunst
Oct 18, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Michael P Walsh, William C Cole
Feb 20, 2015·Canadian Journal of Physiology and Pharmacology·Jena Fediuk, Shyamala Dakshinamurti
Aug 4, 2009·Journal of Biomechanics·S RoyN Stergiopulos
Mar 11, 2017·Frontiers in Pharmacology·Ahmed F El-Yazbi, Khaled S Abd-Elrahman
Mar 23, 2017·Journal of Cardiovascular Pharmacology·Nicholas A Flavahan
Mar 13, 2017·Free Radical Biology & Medicine·Qian XuKathy K Griendling
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·George J C YeKevin Kit Parker
May 5, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Philip S CliffordMichael A Hill
Dec 6, 2006·Hypertension·Egidius H J HeerkensAnthony M Heagerty
Jan 21, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Li HaoCarlos Fernandez-Patron
Aug 25, 2015·BJOG : an International Journal of Obstetrics and Gynaecology·Z JallahP Moalli
Mar 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Michael A Hill, Michael J Davis
Sep 20, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jena FediukShyamala Dakshinamurti
Jan 10, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Ursula StorchThomas Gudermann
Jan 31, 2012·Journal of Cellular Physiology·Jianghong MinKathleen G Morgan
Jun 13, 2012·The Journal of Physiology·Rina Yamin, Kathleen G Morgan
Jun 22, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Sheila Flavahan, Nicholas A Flavahan
Dec 19, 2009·American Journal of Physiology. Cell Physiology·Hak Rim KimKathleen G Morgan
Jun 23, 2009·Trends in Pharmacological Sciences·Michael A HillIsmail Laher

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