Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy

Biophysical Journal
Andrew I ShevchukYuri E Korchev

Abstract

We have developed a high-resolution scanning surface confocal microscopy technique capable of imaging single virus-like particles (VLPs) on the surfaces of cells topographically and by fluorescence. The technique combines recently published single-molecule-resolution ion-conductance microscopy that acquires topographical data with confocal microscopy providing simultaneous fluorescent imaging. In our experiments we have demonstrated that the cell membrane exhibits numerous submicrometer-sized surface structures that could be topographically confused with virus particles. However, simultaneous acquisition of confocal images allows the positions of fluorescently tagged particles to be identified. Using this technique, we have, for the first time, visualized single polyoma VLPs adsorbed onto the cell membrane. Observed VLPs had a mean width of 108 +/- 16 nm. The particles were randomly distributed across the cell membrane, and no specific interactions were seen with cell membrane structures such as microvilli. These experiments demonstrate the utility of this new microscope for imaging the interactions of nanoparticles with the cell surface to provide novel insights into the earliest interactions of viruses and other nanoparticles...Continue Reading

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Citations

May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yasufumi TakahashiTomokazu Matsue
Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David KlenermanSimon J Davis
Dec 4, 2012·Sensors·Patrick HappelIrmgard D Dietzel
Dec 21, 2013·Nanomedicine·Yang GaoYury Gogotsi
Apr 25, 2012·Annual Review of Analytical Chemistry·Chiao-Chen ChenLane A Baker
May 21, 2010·Physical Chemistry Chemical Physics : PCCP·Yasufumi TakahashiTomokazu Matsue
Jun 22, 2010·The Analyst·Celeste A MorrisLane A Baker
Oct 23, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Max J LabJulia Gorelik
Jul 20, 2012·Physiological Reviews·Elise BalseStéphane N Hatem
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Joanna BednarskaAndrew Shevchuk
Oct 31, 2018·Biosensors·Marta Maria Pereira da Silva Neves, Daniel Martín-Yerga
May 14, 2009·Analytical Chemistry·Chiao-Chen ChenLane A Baker
Dec 26, 2017·ACS Sensors·Jingjing ZhangHong-Yuan Chen
Dec 10, 2020·Chemical Reviews·Cheng ZhuLane A Baker

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