Imaging spectrometer fundamentals for researchers in the biosciences--a tutorial

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Jeremy M Lerner

Abstract

Over the last 2 years there has been a dramatic increase in the number of bioscience laboratories using wavelength dispersive spectroscopy to study in vivo, in situ fluorescence. Transforming spectral information into an image provides a graphic means of mapping localized ionic, molecular, and protein-protein interactions. Spectroscopy also enables fluorophores with overlapping spectral features to be delineation. In this study, we provide the tools that a researcher needs to put into perspective instrumental contributions to a reported spectrum in order to gain greater understanding of the natural emission of the sample. We also show how to deduce the basic capabilities of a spectral confocal system. Finally, we show how to determine the true spectral bandwidth of an object, the illuminated area of a laser-excited object, and what is needed to optimize light throughput.

References

Jan 1, 1992·Journal of Microscopy·P A BenedettiS Vestri
Jan 1, 1986·Journal of Microscopy·R S BalabanP D Smith
Oct 7, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jeremy M Lerner, Robert M Zucker
Jan 1, 2000·Journal of Research of the National Institute of Standards and Technology·M O'BrienT Sander

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Citations

Oct 10, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Richard W ColeClaire M Brown
Sep 1, 2014·The Review of Scientific Instruments·Seo Hyun KimJai Hoon Lee
Jan 26, 2011·Physical Chemistry Chemical Physics : PCCP·Pablo G EtchegoinA Fainstein
Dec 1, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert M ZuckerWilliam K Boyes
Aug 15, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·R M ZuckerW K Boyes
Jun 22, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·R M ZuckerW K Boyes
Oct 28, 2014·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Figen S OktemJoseph M Davila
Sep 26, 2015·Optics Express·Thomas MissbachAndreas W Bett
Feb 16, 2017·Scientific Reports·Natsuki KandaMakoto Kuwata-Gonokami
Jun 8, 2017·Advanced Materials·Hao ZengArri Priimagi
Jun 26, 2010·Current Protocols in Cytometry·Jeremy M LernerElliot Wachman
Mar 22, 2016·Chemical Society Reviews·Isaac Pence, Anita Mahadevan-Jansen
Jan 8, 2013·Current Protocols in Cytometry·John P Nolan, Danilo Condello
Apr 3, 2008·Applied Optics·Ashwin WagadarikarDavid Brady
May 2, 2018·Optics Letters·Cong BaJerome Mertz
Jan 1, 2016·Light, Science & Applications·Stefan SchuhladenHans Zappe
Jun 19, 2019·Polymers·Chuen-Lin TienShuan-Yu Huang
Nov 18, 2021·The Analyst·Mustafa UnalJeffry S Nyman
Jul 9, 2019··Paul PattRagavender M. S

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