Imaging stem cell differentiation for cell-based tissue repair

Methods in Enzymology
Zhenghong LeeArnold Caplan

Abstract

Mesenchymal stem cells (MSCs) can differentiate into a number of tissue lineages and possess great potential in tissue regeneration and cell-based therapy. For bone fracture or cartilage wear and tear, stem cells need to be delivered to the injury site for repair. Assessing engraftment of the delivered cells and their differentiation status is crucial for the optimization of novel cell-based therapy. A longitudinal and quantitative method is needed to track stem cells transplanted/implanted to advance our understanding of their therapeutic effects and facilitate improvements in cell-based therapy. Currently, there are very few effective noninvasive ways to track the differentiation of infused stem cells. A brief review of a few existing approaches, mostly using transgenic animals, is given first, followed by newly developed in vivo imaging strategies that are intended to track implanted MSCs using a reporter gene system. Specifically, marker genes are selected to track whether MSCs differentiate along the osteogenic lineage for bone regeneration or the chondrogenic lineage for cartilage repair. The general strategy is to use the promoter of a differentiation-specific marker gene to drive the expression of an established reporte...Continue Reading

Citations

Oct 3, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Arthur A NeryHenning Ulrich
Feb 27, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·David J CornZhenghong Lee
Jan 29, 2016·Annals of Biomedical Engineering·Joseph M MansourJean F Welter

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