PMID: 11933015Apr 5, 2002Paper

Imaging technique implemented in CellTracks system

Cytometry
Arjan G J TibbeLeon W M M Terstappen

Abstract

We developed the CellTracks cell analysis system that, similar to flow cytometry, yields multiparameter information by which the cells can be differentiated. We describe the implementation of a laser scanning imaging method in the system. Image analysis of the cells improves the specificity of cell classification, especially in cases where the particular cells are found relatively infrequently and one has to discriminate between artifacts and real events. Fluorescent images of immunomagnetically labeled and aligned cells are obtained by passing the cells through a laser focus. The laser focus is smaller than the objects and subsequent frames captured by a regular surveillance CCD camera with a frame grabber board represent different parts of the cells. Complete images of the cells are constructed by shifting each image with respect to each other and adding individual pixel values. The power of combining a fluorescent image with multiparametric data is demonstrated by imaging fluorescent and magnetically labeled beads and cells. The image gives additional information about the dye distribution across the objects. Changes in dye distribution as a function of time were observed in leukocytes labeled with the red fluorescent label,...Continue Reading

References

Dec 10, 1999·Nature Biotechnology·A G TibbeL W Terstappen

❮ Previous
Next ❯

Citations

Mar 14, 2002·Cytometry·Arjan G J TibbeLeon W M M Terstappen
Jun 22, 2012·Journal of Fluorescence·Leo L ChanNing Lai
Aug 9, 2003·Journal of Neuroscience Methods·Jürgen J LorenzJeffery L Barker
Sep 13, 2013·BMC Cancer·Jeffrey CummingsCaroline Dive
May 28, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gerhardt AttardJohann S de Bono
Jul 30, 2011·Trends in Biotechnology·David S BoyleXuanhong Cheng
Mar 15, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christian PaarAlois Sonnleitner
Jan 13, 2006·Journal of Cellular Biochemistry·Vladimir P ZharovValery V Tuchin
Feb 27, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Vladimir P ZharovValery V Tuchin
Mar 1, 2008·Cytometry. Part B, Clinical Cytometry·F MandyS Faucher
Dec 2, 2017·Journal of Neuro-oncology·Mark T J van BusselDieta Brandsma
Jun 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Johann S de BonoAntonio Gualberto
Oct 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Paul HaluskaJohann S de Bono

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.