Imaging transplanted stem cells in real time using an MRI dual-contrast method

Scientific Reports
Ethel J NgenDmitri Artemov

Abstract

Stem cell therapies are currently being investigated for the repair of brain injuries. Although exogenous stem cell labelling with superparamagnetic iron oxide nanoparticles (SPIONs) prior to transplantation provides a means to noninvasively monitor stem cell transplantation by magnetic resonance imaging (MRI), monitoring cell death is still a challenge. Here, we investigate the feasibility of using an MRI dual-contrast technique to detect cell delivery, cell migration and cell death after stem cell transplantation. Human mesenchymal stem cells were dual labelled with SPIONs and gadolinium-based chelates (GdDTPA). The viability, proliferation rate, and differentiation potential of the labelled cells were then evaluated. The feasibility of this MRI technique to distinguish between live and dead cells was next evaluated using MRI phantoms, and in vivo using both immune-competent and immune-deficient mice, following the induction of brain injury in the mice. All results were validated with bioluminescence imaging. In live cells, a negative (T2/T2*) MRI contrast predominates, and is used to track cell delivery and cell migration. Upon cell death, a diffused positive (T1) MRI contrast is generated in the vicinity of the dead cells, ...Continue Reading

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Citations

Nov 7, 2016·Molecular Neurobiology·Ajay KumarBalázs Gulyás
Jan 21, 2017·International Journal of Molecular Sciences·Ethel J Ngen, Dmitri Artemov
Aug 15, 2017·Current Protocols in Stem Cell Biology·Ethel J NgenDmitri Artemov
Aug 3, 2017·Molecular Medicine Reports·Yanhui ZhangZongwu Deng
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Rasoul YahyapourElahe Motevaseli
Apr 14, 2017·Journal of the Royal Society, Interface·Sabine van Rijt, Pamela Habibovic
Nov 9, 2018·Scientific Reports·Izabela Malysz-CymborskaPiotr Walczak
Jan 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ana F AlmeidaManuela E Gomes
Oct 16, 2020·Journal of Stroke·Shuai ZhangXiaofeng Jia
Jul 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yanhui ZhangZongwu Deng
Jul 12, 2016·Molecular Pharmaceutics·Ethel J NgenAssaf A Gilad
Oct 16, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Shehzahdi Shebbrin MoonshiHang Thu Ta

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Methods Mentioned

BETA
imaging techniques
transfection
PCR
X-ray

Software Mentioned

IDL
Xenogen Living Imaging
NIH ImageJ
Amira
Paravision
Exelis
Exelis Visual

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