Imatinib Ameliorated Retinal Neovascularization by Suppressing PDGFR-α and PDGFR-β

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Lingli ZhouXialin Liu

Abstract

Platelet-derived growth factors (PDGFs) have emerged as pivotal in pathological angiogenesis, which is a hallmark of various tumors and retinal diseases. Here we evaluated the anti-angiogenic effect of imatinib, an inhibitor of PDGF receptors α and β (PDGFR-α and -β), in retinal neovascularization using an oxygen-induced retinopathy (OIR) model. The OIR model was established and given imatinib or vehicle treatments daily from P12 to P16. At the peak of angiogenesis at P17, the neovascularization area was quantified on retinal whole-mounts with isolectin B4 staining. Immunofluorescence staining and western blots were used to determine the effect of imatinib on different vascular cells and the pathway molecules involved. Imatinib effectively suppressed pathological angiogenesis in OIR mice and reduced the number of all three types of vascular cells, including endothelial cells, pericytes, and smooth muscle cells. Moreover, the expression and activation of PDGFR-α and -β were inhibited by imatinib. The imatinib-treated OIR mice presented with reduced expression of other potent pro-angiogenic factors such as VEGF and FGF2. No obvious retinal or systemic side effects were observed in the imatinib treatment group. Imatinib appears to...Continue Reading

Citations

Aug 4, 2020·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Sihong ShenWeining Hu
Apr 9, 2020·International Journal of Molecular Sciences·Tamás Kovács-ÖllerBotir T Sagdullaev
Oct 26, 2020·Diabetologia·Yohei TomitaYoshihiko Usui
Jan 29, 2021·International Journal of Molecular Sciences·Nundehui Díaz-LezamaAntje Grosche
Apr 26, 2021·Pediatric Research·Hanna DanielssonAnn Hellström

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