Abstract
The use of molecular target therapy has not been established for endometrial cancer. The present study investigated the potential therapeutic strategy of targeting CD117-positive cancer cells as a novel molecular target therapy. FACS-sorted CD117(+) cells isolated from endometrial cancer cell lines (Ishikawa or MFE280 cells) exhibited higher proliferative capacity in vitro and colony forming activity on soft agar, and decreased sensitivity to cisplatin, compared to CD117(-) cells. Immunohistochemical analyses with surgical specimens of endometrial cancers showed that high CD117 expression was tightly linked to advanced FIGO stages, myometrial invasion and histological grade, and was significantly associated with poor overall survival and relapse-free survival (Kaplan-Meier analysis; p<0.001, log-rank test). The Cox-regression hazard model identified high CD117 expression to be an independent prognostic factor for survival (p<0.05). In vitro assay confirmed that stem cell factor (SCF), a ligand of CD117, was produced specifically in CD117(+) cells of endometrial cancer, and the colony-forming activity were abrogated by adding anti-SCF antibody, indicating an SCF-dependent growth property. Imatinib was confirmed to selectively ta...Continue Reading
References
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·W E CarsonM A Caligiuri
Apr 22, 2003·Pathology Oncology Research : POR·Chandu M V de Silva, Robin Reid
Feb 27, 2004·The Journal of Biological Chemistry·Junji MatsuiMasayuki Okada
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·L Rönnstrand
Dec 29, 2004·Leukemia & Lymphoma·Lidia LarizzaAlessandro Beghini
Sep 17, 2005·Cancer Research·Alan McIntyreJanet Shipley
Apr 18, 2006·Cancer Cell·Lixin SunHoward A Fine
Jul 5, 2006·Molecular Cancer Therapeutics·Julie Litz, Geoffrey W Krystal
Jul 6, 2006·Journal of Cell Science·Anu BashambooLesley M Forrester
Dec 16, 2006·Gynecologic Oncology·Tanya J Shaw, Barbara C Vanderhyden
Dec 30, 2006·Seminars in Diagnostic Pathology·Jerzy Lasota, Markku Miettinen
Mar 14, 2007·The International Journal of Biochemistry & Cell Biology·Charlotte E Edling, Bengt Hallberg
Apr 6, 2007·Digestive Diseases and Sciences·Akira YasudaTadao Manabe
Jun 15, 2007·Experimental Hematology·Min LuCheryl D Helgason
Jun 4, 2008·Cancer Research·Shu ZhangKenneth P Nephew
Jul 22, 2008·Leukemia Research·H T Hassan
Dec 24, 2009·Cancer Research·Vera LevinaElieser Gorelik
Jan 7, 2010·The Journal of Clinical Investigation·Christophe GinestierMax S Wicha
Oct 29, 2010·Journal of Oncology·Ryan MorrisonBo Lu
Mar 6, 2012·Gynecologic Oncology·Ignacio Romero NogueraDavid M Gershenson
Citations
Dec 25, 2015·Human Mutation·Min ZhaoTracy A O'Mara
Nov 8, 2014·PloS One·Ruixia HuangZhenhe Suo
Jul 10, 2017·Clinical and Translational Science·S HuA Sparreboom
Nov 23, 2019·Cancers·Gaia GiannoneGiorgio Valabrega
Jun 28, 2016·Oncotarget·Ruixia Huang, Einar K Rofstad
Apr 13, 2020·Cancer Science·Kai ZengYue Zhao
Jan 19, 2019·Oncology Letters·Shiqin MaoHong Yu
Aug 14, 2020·Life Sciences·JinHui LiuWenJun Cheng
Aug 8, 2021·International Journal of Molecular Sciences·Tomohiko FukudaCarl-Henrik Heldin
Aug 19, 2021·Molecular Oncology·Jin-Ock KimSang Gyu Park