IMCD cells cultured from Dahl S rats absorb more Na+ than Dahl R rats

The American Journal of Physiology
R F HustedJohn B Stokes

Abstract

Dahl salt-sensitive (S) rats develop hypertension in response to a high-salt diet, whereas Dahl salt-resistant (R) rats do not. There is good evidence that the Dahl S kidneys have diminished natriuretic capacity. We studied the rate of Na+ transport by primary cultures of the inner medullary collecting duct from these two strains to determine whether there were intrinsic differences. Monolayers obtained from prehypertensive S rats transported Na+ at twice the rate as monolayers from age-matched R rats. Mineralocorticoid and glucocorticoid hormones increased Na+ transport from both strains; the S rat monolayers always displayed higher transport rates than R rat monolayers with the same treatment. The Na+ entry pathway in both S and R rat monolayers was via an Na+ channel. The difference in Na+ transport was not explained by a difference in the metabolism of corticosterone, ATP content, citrate synthase activity, ultrastructural appearance, or rate of maturation. Monolayers from S rats tended to have higher protein and DNA content, but these differences could not account for the difference in Na+ transport. Anion secretion in response to adenosine 3',5'-cyclic monophosphate agonists was similar. These results demonstrate intrinsi...Continue Reading

Citations

Jun 7, 2006·Current Opinion in Cardiology·Bing S HuangFrans H H Leenen
Oct 13, 2010·Annual Review of Physiology·Jeffrey L GarvinPablo A Ortiz
Aug 1, 2012·Physiological Research·J ZichaJ Kuneš

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