Imino acids and collagen triple helix stability: characterization of collagen-like polypeptides containing Hyp-Hyp-Gly sequence repeats

Journal of the American Chemical Society
Rita BerisioAdriana Zagari

Abstract

The analysis of factors contributing to the stability of proteins is a subject of intense debate. Particularly challenging is the study of structural proteins, since their function is their structure. Among these is collagen, the key structural component of bones, skin, cartilage, tendons, and other connecting tissues. It is well established that the collagen triple helix is characterized by the presence of hydroxyproline, whose content modulates triple helix thermal stability according to the requirement of the host organism. Because of the complexity and the fibrous nature of collagen, data on the stability and structure of this protein have been mainly obtained by the use of collagen-like polypeptides. On the basis of CD characterization of collagen-like polypeptides we here show that the presence of Hyp at the X position of repeating triplets Hyp-Hyp-Gly stabilizes the triple helix significantly. This extra-stabilization has been ascribed, by using molecular modeling, to the formation of a hydrogen bond between Hyp residues belonging to the X and the Y positions of adjacent chains. This communication also provides a comprehensive interpretation of the ensemble of available data on polypeptides containing proline derivatives.

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Citations

Apr 13, 2011·The Journal of Biological Chemistry·Matthew D Shoulders, Ronald T Raines
Aug 27, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Layla AzamJ Michael McIntosh
Apr 7, 2009·Annual Review of Biochemistry·Matthew D Shoulders, Ronald T Raines
Jan 12, 2013·Current Protein & Peptide Science·Rita Berisio, Luigi Vitagliano
Dec 24, 2013·Applied Microbiology and Biotechnology·Christoph RutschmannThierry Hennet
Sep 12, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Punitha VelmuruganAruna Dhathathreyan
May 20, 2008·Biochemical and Biophysical Research Communications·Alfonso De SimoneRita Berisio
Dec 5, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Rita BerisioLuigi Vitagliano
Mar 29, 2008·Protein Science : a Publication of the Protein Society·Roberto ImprotaLuigi Vitagliano
May 5, 2011·Biophysical Journal·Luigi VitaglianoAlfonso De Simone
Apr 20, 2005·Advances in Protein Chemistry·Barbara Brodsky, Anton V Persikov
Jun 2, 2005·Chembiochem : a European Journal of Chemical Biology·Nattha JiravanichanunTeresita Silva
Apr 18, 2012·Macromolecular Rapid Communications·Hideharu Mori, Takeshi Endo
Sep 6, 2018·Current Medicinal Chemistry·Dudley H McNittSlawomir Lukomski
Apr 24, 2012·Organic & Biomolecular Chemistry·Sayani ChattopadhyayRonald T Raines
Nov 16, 2017·Protein Science : a Publication of the Protein Society·Flavia SquegliaRita Berisio
May 10, 2020·Molecular & Cellular Proteomics : MCP·Alexander R OckenSarah Calve
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Alexander R OckenSarah Calve
Feb 14, 2006·The Journal of Physical Chemistry. B·Il Keun Song, Young Kee Kang
Oct 4, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Shuizhen ZongHuangxian Ju
Nov 10, 2005·Journal of the American Chemical Society·Jonathan A Hodges, Ronald T Raines

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