Imipenem resistance in clinical Escherichia coli from Qom, Iran

BMC Research Notes
Saeed ShamsAlessandra Piccirillo

Abstract

The emergence of metallo-β-lactamase-producing Enterobacteriaceae is a worldwide health concern. In this study, the first evaluation of MBL genes, bla IMP and bla VIM , in Escherichia coli resistant to imipenem isolated from urine and blood specimens in Qom, Iran is described. Three hundred urine and blood specimens were analysed to detect the presence of E. coli. Resistance to imipenem and other antimicrobials was determined by disk diffusion and MIC. MBL production was screened using CDDT. PCR was also carried out to determine the presence of bla IMP and bla VIM genes in imipenem-resistant isolates. In total, 160 E. coli isolates were collected from March to May 2016. According to disk diffusion, high-level of resistance (20%) to cefotaxime was observed, whereas the lowest (1%) was detected for tetracycline. In addition, five isolates showed resistance to imipenem with a MIC ≥ 4 µg/mL. CDDT test confirmed that five isolates were MBL-producing strains, but no bla IMP and bla VIM genes were detected. Results of this study show a very low level of resistance to imipenem in our geographical area.

References

Nov 14, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G CornagliaR Fontana
Nov 3, 2001·International Journal of Antimicrobial Agents·Y SáenzC Torres
Jan 16, 2007·International Journal of Antimicrobial Agents·Giuseppe CornagliaUNKNOWN ESCMID Study Group for Antimicrobial Resistance Surveillance (ESGARS)
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Dec 10, 2009·Antimicrobial Agents and Chemotherapy·Karen Bush, George A Jacoby
Mar 10, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C WendtD Jonas
Jul 6, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C G GiskeN Woodford
Sep 18, 2010·Diagnostic Microbiology and Infectious Disease·Saber YousefiNima Hosseini Jazani
May 3, 2011·The Lancet Infectious Diseases·Giuseppe CornagliaGian Maria Rossolini
Oct 15, 2011·Journal of Clinical Microbiology·Brandi M LimbagoAlex J Kallen
Apr 2, 2013·Diagnostic Microbiology and Infectious Disease·Norifumi ShigemotoMotoyuki Sugai
Jan 7, 2014·Diagnostic Microbiology and Infectious Disease·Gisele PeiranoJohann D D Pitout
Oct 1, 2015·Jundishapur Journal of Microbiology·Muhammad SohailAbdul Arif Khan
Nov 16, 2017·Acta Microbiologica Et Immunologica Hungarica·Aghil BahramianMohsen Heidary

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.