Immature Exosomes Derived from MicroRNA-146a Overexpressing Dendritic Cells Act as Antigen-Specific Therapy for Myasthenia Gravis

Inflammation
Weifan YinHuan Yang

Abstract

Myasthenia gravis (MG) is a neurological autoimmune disease characterized by fluctuating weakness of certain voluntary muscles. Current treatments for MG are largely directed at suppressing the whole immune system by using immunosuppressants or glucocorticoids and often cause several side effects. The ideal therapeutic methods for MG should suppress aberrant immunoactivation specifically, while retaining normal function of the immune system. In this study, we first produced exosomes from microRNA-146a overexpressing dendritic cells (DCs). Then, we observed suppressive effects of those exosomes in experimental autoimmune myasthenia gravis (EAMG) mice. Results showed that exosomes from microRNA-146a overexpressing DCs expressed decreased levels of CD80 and CD86. In experimental autoimmune MG, exosomes from microRNA-146a overexpressing DCs suppressed ongoing clinical MG in mice and altered T helper cell profiles from Th1/Th17 to Th2/Treg both in serum and spleen, and the therapeutic effects of those exosomes were antigen-specific and partly dose dependent. All the findings provide experimental basis for antigen-specific therapy of MG.

References

Jun 23, 1994·The New England Journal of Medicine·D B Drachman
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·W P MinM S Cattral
Jan 19, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·P ChristadossC Deng
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hiroyuki MatsueAkira Takashima
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seon-Hee KimPaul D Robbins
Jul 2, 2005·Blood Cells, Molecules & Diseases·Elodie SeguraClotilde Théry
Nov 9, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Seon Hee KimPaul D Robbins
Jul 11, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H PêcheM C Cuturi
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seon Hee KimPaul D Robbins
Jan 26, 2010·Nature Reviews. Immunology·Ryan M O'ConnellDavid Baltimore
Mar 25, 2011·Methods in Molecular Biology·Pablo Andrés Manavella, Ignacio Rubio-Somoza
Sep 4, 2012·Molecular Medicine Reports·Jing XiaoJing Xiong
Nov 6, 2012·Molecular Cell·Takashi Fukaya, Yukihide Tomari
Jun 14, 2013·Clinical & Developmental Immunology·Chun Yuen J ChungNathalie Cools
Nov 28, 2013·Journal of Neuroimmunology·Aya D PusicRichard P Kraig
Aug 27, 2014·Muscle & Nerve·Tomihiro ImaiKimiaki Utsugisawa
Oct 8, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jie Luo, Jon Lindstrom
Apr 17, 2015·Clinical Journal of the American Society of Nephrology : CJASN·Bruce M Hall
Jul 23, 2015·Journal of Neuroimmunology·Ning BuHong-Wei Ren
Nov 6, 2015·Frontiers in Immunology·Fabiola OsorioFermín E González
Apr 2, 2016·The Journal of Clinical Investigation·Jonathan M PittLaurence Zitvogel

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Citations

Aug 30, 2018·Current Opinion in Neurology·Renato MantegazzaPaola Cavalcante
Nov 13, 2018·Journal of Cellular Physiology·Soheila MoeinBahman Yousefi
Jul 28, 2018·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·S JiangM Lu
Jun 9, 2020·Frontiers in Neuroscience·Lin Wang, Lijuan Zhang
Nov 11, 2017·Annals of the New York Academy of Sciences·Neil J CobelliHui B Sun
Apr 16, 2019·Frontiers in Immunology·Runda WuJunbo Ge
Apr 18, 2019·International Journal of Molecular Sciences·Stefania RaimondoRiccardo Alessandro
Jan 13, 2019·International Journal of Molecular Sciences·Claudia CampanellaStefano Fais
Nov 8, 2020·International Journal of Rheumatic Diseases·Alireza HejratiParisa Zafari
Mar 17, 2021·Autoimmunity Reviews·Rui ZhaoChongbo Zhao
May 5, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rih-Yang HuangChien-Wen Chang
Jul 6, 2021·Journal of Neuroimmunology·Rasoul MirzaeiSajad Karampoor

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Methods Mentioned

BETA
transfection
ELISA
FACS
protein assay
electrophoresis

Software Mentioned

SPSS

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