Immature megakaryocytes in the mouse: in vitro relationship to megakaryocyte progenitor cells and mature megakaryocytes

Journal of Cellular Physiology
M W LongT P McDonald

Abstract

An assay describing conditions for the maturation of single immature megakaryocytes in vitro is reported. Enriched populations of small, relatively immature megakaryocytes have been found to develop into single, mature megakaryocytes by 60 hours in semisolid agar cultures. Continued incubation of these cells did not lead to the formation of colonies within 5-7 days. Maturation was indicated by increasing cell size and cytoplasmic and acetylcholinesterase content. Factors stimulating the development of immature megakaryocytes were found in preparations of human embryonic kidney cell-conditioned media (a source of in vivo Thrombopoietic Stimulatory Factor), peritoneal exudate cell-conditioned medium, lung-conditioned medium, or bone marrow cellular sources of activity (adherent cells or cells that sediment at 5-6 mm hr-1). Immature megakaryocytes cultured serum free responded to sources of an auxiliary megakaryocyte potentiating activity by developing into single, large megakaryocytes but did not respond to a megakaryocyte colony-stimulating factor devoid of detectable potentiator activity present in WEH1-3-conditioned medium. In contrast, serum-free proliferation of the megakaryocyte progenitor cell required both megakaryocyte c...Continue Reading

References

Jun 1, 1976·The Journal of Experimental Medicine·P RalphK Nilsson
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D MetcalfB Sordat
Mar 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·A Nakeff, S Daniels-McQueen
Jun 1, 1969·Journal of Cellular Physiology·R G Miller, R A Phillips
Jan 1, 1982·Journal of Cellular Physiology·N WilliamsD J Nelson
Jan 1, 1981·Cell and Tissue Kinetics·M H FreedmanE F Saunders
Oct 1, 1954·Acta Haematologica·J ZAJICEK
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS

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Citations

Nov 1, 1993·Stem Cells·H Avraham
Jan 1, 1990·Biotherapy·A GrossiP Rossi Ferrini
Aug 1, 1995·Critical Reviews in Oncology/hematology·W VainchenkerF Wendling
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T IshibashiS A Burstein
Nov 1, 1984·The Journal of Clinical Investigation·M W LongL A Boxer
Nov 1, 1988·The Journal of Clinical Investigation·M W LongS G Emerson
Dec 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thanyaphong Na NakornIrving L Weissman
Aug 1, 2007·Journal of Thrombosis and Haemostasis : JTH·Y ChangW Vainchenker
Jul 22, 2006·British Journal of Haematology·Varda R Deutsch, Aaron Tomer
Nov 1, 1984·British Journal of Haematology·D A LeporeD G Penington
Apr 1, 1989·British Journal of Haematology·A GrossiP Rossi Ferrini
Apr 1, 1984·British Journal of Haematology·G VinciW Vainchenker
May 1, 1989·International Journal of Cell Cloning·T P McDonald
Jul 1, 1989·Baillière's Clinical Haematology·A EldorR F Levine
Jan 1, 1990·Nouvelle Revue Française D'hématologie·Z C HanJ P Caen
Jan 1, 1987·Annals of the New York Academy of Sciences·T P McDonald
Jan 1, 1989·Annals of the New York Academy of Sciences·M W Long
Jan 1, 1989·Baillière's Clinical Haematology·A L Jones, J L Millar

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