PMID: 8596728Mar 1, 1996Paper

Immature properties of large-conductance calcium-activated potassium channels in rat neuroepithelium

Pflügers Archiv : European journal of physiology
J M Mienville, J L Barker

Abstract

The pharmacological and biophysical properties of large-conductance Ca-activated K (BK) channels from embryonic rat telencephalic neuroepithelium were investigated with in situ patch-clamp techniques. A fraction of these channels exhibited properties characteristic of BK channels recorded in well differentiated cells, including normal gating mode (BKN channels). The vast majority of BK channels expressed distinctive properties, the most conspicuous being their buzz gating mode (BKB channels). BKB channels were insensitive to a concentration of charybdotoxin that completely and reversibly blocked BKN channels. In contrast with the strict dependence of BKN channel activation on cytoplasmic Ca, BKB channels displayed substantially high open probability (Po) after inside-out patch excision in a Ca-free medium. Intracellular trypsin down-regulated the Po of BKB channels, which then exhibited a greater sensitivity to cytoplasmic Ca, mainly in the positive direction (increased Po with increased Ca). This suggested a modulatory role for Ca as opposed to its gating role in BKN channels. Ca ions also reduced current amplitude of both types of channels. BKB channels were less voltage sensitive than BKN channels, but this was not correlate...Continue Reading

References

Apr 1, 1992·The American Journal of Physiology·L SalomaoS G Schultz
Aug 1, 1991·Journal of Bioenergetics and Biomembranes·O B McManus
Jan 1, 1991·The Japanese Journal of Physiology·A YoshidaY Ikemoto
Mar 1, 1991·The American Journal of Emergency Medicine·M J Bayer
Jan 1, 1989·Neuron·P H ReinhartI B Levitan
Jan 1, 1989·Annual Review of Genetics·C W SmithB Nadal-Ginard
Sep 1, 1989·Pflügers Archiv : European journal of physiology·F A EdwardsT Takahashi
Nov 1, 1988·Pflügers Archiv : European journal of physiology·P D BregestovskiV Zamoyski
Jan 1, 1994·Developmental Neuroscience·E J BulanJ M Mienville
Jan 22, 1993·Proceedings. Biological Sciences·R N KhanM L Ashford
Jan 14, 1994·Brain Research. Developmental Brain Research·J M MienvilleJ L Barker
Jan 15, 1993·Brain Research. Developmental Brain Research·A K ChakrabartiE L Hogan

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Citations

Aug 7, 1998·Brain Research. Developmental Brain Research·A I SantosP F Costa
Sep 23, 1997·Neuroscience·J M Mienville, J L Barker
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·P MeeraL Toro
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·P Subramony, S E Dryer
Oct 20, 1998·Journal of Neurobiology·W J Moody

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