Immediate and delayed (after cooling) effects of centrifugation on equine sperm

Theriogenology
J A LenG Hosgood

Abstract

The objectives of this study were to determine the effects of centrifugation on equine sperm total and progressive motility, viability, and acrosomal integrity. We hypothesized that although high centrifugation forces would be detrimental to equine Equus caballus sperm, recovery rates would increase. Ejaculates from six stallions were collected, extended to a concentration of 25x10(6) cells/mL, and subjected for 10min to (1) no centrifugation (NC) or (2) centrifugation at 400xg, (3) 900xg, or (4) 4500xg. Before and after centrifugation (Day 0), and after 24h of cooling (Day 1), sperm motility was assessed by computer-assisted semen analysis, and samples were stained with SYBR-14/propidium iodide (PI) for viability and with PI/fluorescein isothiocyanate (FITC)-Peanut aglutinin (PNA) (Arachis hypogaea) for acrosomal integrity. The effect of treatment and day on motility, viability, and acrosomal integrity was determined using a mixed linear model. Compared with the other treatments, centrifugation at 4500xg reduced all end points measured (P<0.05). Both 400xg and 900xg yielded lower recovery rates than that of 4500xg (NC=100.0+/-0.0%; 400xg=54.4+/-8.6%; 900xg=75.0+/-7.1%; 4500xg=97.9+/-2.8%; P<0.05). Centrifugation at 400xg or 90...Continue Reading

References

Jan 1, 1996·Equine Veterinary Journal·A V HeitlandC Hamilton
Jan 1, 1995·Reproduction, Fertility, and Development·R J Aitken
Jul 19, 2000·Archives of Biochemistry and Biophysics·A Abou-Haila, D R Tulsiani
Jun 26, 2004·Human Reproduction Update·W C L Ford
May 25, 2005·Theriogenology·A I MooreJ K Graham
Aug 6, 2005·Animal Reproduction Science·Christine Aurich
Jan 20, 2006·Reproduction in Domestic Animals = Zuchthygiene·A M KareskoskiT Katila

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Citations

Dec 14, 2011·Equine Veterinary Journal·M K HoogewijsA van Soom
Apr 4, 2018·Reproduction, Fertility, and Development·Daznia BompartCarles Soler
Oct 14, 2018·Reproduction in Domestic Animals = Zuchthygiene·Jose A LenSara K Lyle
Nov 6, 2021·Acta Veterinaria Scandinavica·Marta Neila-MonteroLuis Anel

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