Immediate and gradual gene expression changes in telomerase over-expressing fibroblasts

Biochemical and Biophysical Research Communications
Darren J DanielsG Saretzki

Abstract

Most human somatic cells contain no or very low levels of telomerase. The over-expression of the catalytic subunit (hTERT) of human telomerase is a common method to generate cells with a greatly prolonged lifespan. These cells serve as models for cells that are either difficult to cultivate or have a limited lifespan in vitro. In addition, hTERT over-expressing cells are thought to be a useful resource for tissue engineering and regenerative medicine. While tumour suppressors and cell cycle checkpoints are maintained for an extended period in most hTERT over-expressing cells we found that there is a gradual change in gene expression over a range of 130 population doublings (PD) for the majority of genes analysed. Seven genes were significantly down-regulated with increasing population doublings (PDs), while only two were up-regulated. One gene, stanniocalcin 2, was highly expressed in parental fibroblasts but completely diminished as a consequence of hTERT transgene expression. These data demonstrate that in hTERT over-expressing cells two different types of expression changes occur: one can be directly associated with hTERT transgene expression itself, while others might occur more gradual and with varying kinetics. These chan...Continue Reading

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Citations

Jul 7, 2012·International Journal of Radiation Biology·Erta Kalanxhi, Jostein Dahle
Mar 28, 2018·International Journal of Molecular Sciences·Matthew CaleyPhil Stephens
Dec 22, 2019·International Journal of Molecular Sciences·Alexander MartensGabriele Saretzki

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