Immediate cooling does not prevent the ex vivo hydrolysis of L-asparagine by asparaginase

Therapeutic Drug Monitoring
Claudia Lanvers-KaminskyJoachim Boos

Abstract

Monitoring of asparagine (ASN) during asparaginase (ASE) treatment directly links to the antileukemic effect of ASE but is challenging because of ASE-induced ex vivo hydrolysis of ASN. Assuming that ASE is not active at 4°C, immediate cooling of blood samples became the accepted method for ASN determination during ASE therapy. To evaluate the effect of immediate sample cooling on the ex vivo hydrolysis of ASN by ASE the degradation of C4-ASN in whole blood, spiked with different ASE concentrations were analyzed HPLC-MS. C4-ASN and ASE were added either to blood at 4°C or to blood at 37°C, which was instantly cooled down to 4°C. Immediate cooling did not prevent the ex vivo hydrolysis of ASN by ASE. The rate of ASN degradation to aspartic acid depended on the amount of ASE, ASE preparation, and time. Spiked into blood at 4°C 100 U/L native E. coli ASE already immediately degraded 100% of C4-ASN, whereas 10 U/L reduced the amount of C4-ASN by 30%. Spiked into blood at 37°C, which was immediately cooled thereafter, 10 U/L native E. coli ASE hydrolyzed 60% of C4-ASN and 1 U/L between 5% and 10% of C4-ASN. Concentrations of aspartic acid increased in parallel with ASN degradation. In addition, the ex vivo hydrolysis also affected co...Continue Reading

References

Jul 1, 1994·Journal of Chromatography. B, Biomedical Applications·D GentiliM D'Incalci
Sep 1, 1996·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D GentiliG Masera
Aug 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J BoosH Jürgens
Jan 29, 2010·Pediatric Blood & Cancer·Dominik SchreyJoachim Boos

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Citations

Jun 23, 2015·American Journal of Hematology·Mathilde Hunault-BergerUNKNOWN Group for Research on Adult Acute Lymphoblastic Leukemia (GRAALL)
Jul 1, 2017·Pediatric Blood & Cancer·Louise Tram HenriksenUNKNOWN Nordic Society of Pediatric Hematology and Oncology, NOPHO Group
Mar 7, 2019·Journal of Cancer Research and Clinical Oncology·Alexander KarachunskiyUNKNOWN ALL-MB study group
Aug 29, 2020·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·María M Viña-RomeroFernando Gutiérrez-Nicolás
Jan 11, 2020·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robin Q H KloosInge M van der Sluis
Feb 16, 2017·Cancer Chemotherapy and Pharmacology·Claudia Lanvers-Kaminsky
Apr 14, 2021·Journal of Oncology Pharmacy Practice : Official Publication of the International Society of Oncology Pharmacy Practitioners·Wing H Tong
Sep 2, 2021·Cancer Chemotherapy and Pharmacology·Wing H Tong
Dec 10, 2020·Scientific Reports·Daiane Keller CecconelloMariana Bohns Michalowski

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