Immediate-early expression of the herpes simplex virus type 1 ICP27 transcript is not critical for efficient replication in vitro or in vivo

Journal of Virology
Aixu SunEdward K Wagner

Abstract

We constructed a promoter mutation altering the immediate-early expression of the herpes simplex virus type 1 (HSV-1) ICP27 transcript and its cognate wild-type rescue viruses in order to assess the role of the ICP27 protein in the earliest stages of viral infection by global transcriptional analysis with a DNA microarray. This mutant, ICP27/VP16, replaces the whole ICP27 promoter/enhancer with the VP16 promoter. It demonstrates loss of immediate-early expression of ICP27 according to the criteria expression in the absence of de novo protein synthesis and earliest expression in the kinetic cascade. Significant differences in relative transcript abundances between the mutant and wild-type rescue viruses were limited at the earliest times measured and not evident at all by 4 h after infection. Consistent with this observation, levels of some critical proteins were reduced in the mutant as compared to rescue virus infections at the earliest times tested, but were equivalent by 8 h postinfection. Further, both single and multistep levels of virus replication were equivalent with both mutant and rescue viruses. Thus, altering the immediate-early kinetics of ICP27 leads to a suboptimal quantitative lag phase in gene expression but wi...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N Michael, B Roizman
Sep 1, 1985·Journal of Virology·W R SacksP A Schaffer
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P BeardL I Pizer
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·A C WilsonW Herr
Dec 11, 1993·Nucleic Acids Research·D N ArnostiB F Luisi
Jul 1, 1997·Clinical Microbiology Reviews·E K Wagner, D C Bloom
Oct 17, 1998·Methods : a Companion to Methods in Enzymology·E K WagnerM Rice
Feb 14, 2002·Journal of Cellular Biochemistry. Supplement·J Yguerabide, E E Yguerabide
Jul 11, 2002·Progress in Nucleic Acid Research and Molecular Biology·Edward K WagnerPeter Ghazal
Jun 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sara A Jackson, Neal A DeLuca
Jan 1, 1998·Methods in Molecular Medicine·D C Bloom

❮ Previous
Next ❯

Citations

Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Diwakar SanthakumarAmy H Buck
Feb 14, 2006·Journal of Virology·Jing YuanElliott Kieff
Sep 28, 2005·The Journal of General Virology·Peter G E KennedyPeter Ghazal

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.