PMID: 7541880Jul 1, 1995Paper

Immediate-early gene expression in human saphenous veins harvested during coronary artery bypass graft operations

The Journal of Thoracic and Cardiovascular Surgery
R A MoggioG E Reed

Abstract

Saphenous vein graft occlusion is a common late complication of coronary bypass grafting. Intimal smooth muscle cell hyperplasia is a component of this pathobiology, but the underlying molecular events are poorly understood. Immediate-early genes are activated shortly after growth stimulation and subserve cellular functions, which may contribute to intimal smooth muscle cell accumulation. In the present study, human saphenous vein grafts were harvested with minimal manipulation during coronary bypass and processed for isolation of total ribonucleic acid to examine change in immediate-early gene expression of messenger ribonucleic acid by Northern blotting techniques. Thirty saphenous vein grafts were incubated at 4 degrees C in Dulbecco's modified Eagle media from 30 minutes to 10 hours. The messenger ribonucleic acids for immediate-early genes c-fos and c-myc were weak or undetectable in controls but were increased (> 10 times controls) within 1 hour (c-fos) and persisted for at least 6 hours (c-myc) after harvest. Our results demonstrate, for the first time in human vascular tissue, incipient immediate-early gene induction. This information may lead to molecular therapies to control saphenous vein graft disease.

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Citations

Jul 14, 1999·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·M Chikada, M Jones
Jun 1, 1997·The Annals of Thoracic Surgery·H AebertH Schunkert
May 22, 1998·The Annals of Thoracic Surgery·J E O'BrienJ D Mannion
Dec 12, 2013·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Subodh VermaStephen E Fremes
Feb 17, 1998·International Journal of Cardiology·D MehtaG D Angelini
Feb 4, 1999·The Annals of Thoracic Surgery·J D MannionA Zalewski
Sep 26, 2006·The Annals of Thoracic Surgery·Kazuhiro HinokiyamaJarle Vaage
Jan 31, 2012·Current Opinion in Pharmacology·Tatsiana SuvoravaGeorg Kojda
Feb 6, 1998·The Journal of Thoracic and Cardiovascular Surgery·J D MannionA Zalewski

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