Immediate-early protooncogene expression and liver function following various extents of partial hepatectomy in the rat

Digestive Diseases and Sciences
M J MoserG Y Minuk

Abstract

Immediate-early protooncogenes (IEP) are thought to play an important role in hepatocyte replication. Whether the extent of their expression correlates with the strength of the proliferative stimulus and subsequent regenerative activity has yet to be documented in vivo. Data are also lacking with respect to the level at which liver disease is associated with biochemical evidence of hepatic dysfunction. Thus, the objectives of this study were to determine whether a correlation exists between IEP gene mRNA expression and varying extents of partial hepatectomy (PHx) and to document the extent of resection required to result in increases in serum bilirubin levels. Eighty-nine adult, male Sprague-Dawley rats underwent either sham surgery or 20%, 35%, 55%, 70% or 90% PHx. Postoperatively, rats were killed (N = 3-6/group) at 15 and 30 mins and 8 and 24 hrs for c-fos, c-jun, and c-myc mRNA expression by northern blot analyses. Rats killed at 24 hrs also had hepatic regenerative activity documented by [3H]thymidine incorporation into hepatic DNA and serum bilirubin determinations. While c-fos mRNA expression at 15 mins and c-myc mRNA expression at 8 hrs after PHx did not correlate with the extent of PHx (r2 = 0.478 and 0.018, respective...Continue Reading

Citations

Jul 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ian R CorbinGerald Y Minuk
May 11, 2012·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·A Taki-EldinS-S Zheng
Jun 24, 2006·Annals of Surgery·Nodir MadrahimovUta Dahmen
Feb 14, 2007·ANZ Journal of Surgery·Aamir Z Khan, Satvinder S Mudan
Aug 12, 2006·Digestive Diseases and Sciences·Manna ZhangGerald Y Minuk
May 26, 2009·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yuan JiOlaf Dirsch
Aug 12, 2010·Aging Cell·Jingling JinNikolai A Timchenko
Oct 6, 2001·Nitric Oxide : Biology and Chemistry·J M SchoenW W Lautt
Jul 12, 2005·Nitric Oxide : Biology and Chemistry·Jodi M Schoen Smith, W Wayne Lautt
Aug 8, 2021·International Journal of Molecular Sciences·Maxime De RudderIsabelle A Leclercq

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