Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis

Chromosoma
Dmitriy LiAlexander Lorenz

Abstract

Schizosaccharomyces pombe, also known as fission yeast, is an established model for studying chromosome biological processes. Over the years, research employing fission yeast has made important contributions to our knowledge about chromosome segregation during meiosis, as well as meiotic recombination and its regulation. Quantification of meiotic recombination frequency is not a straightforward undertaking, either requiring viable progeny for a genetic plating assay, or relying on laborious Southern blot analysis of recombination intermediates. Neither of these methods lends itself to high-throughput screens to identify novel meiotic factors. Here, we establish visual assays novel to Sz. pombe for characterizing chromosome segregation and meiotic recombination phenotypes. Genes expressing red, yellow, and/or cyan fluorophores from spore-autonomous promoters have been integrated into the fission yeast genomes, either close to the centromere of chromosome 1 to monitor chromosome segregation, or on the arm of chromosome 3 to form a genetic interval at which recombination frequency can be determined. The visual recombination assay allows straightforward and immediate assessment of the genetic outcome of a single meiosis by epi-fluo...Continue Reading

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Citations

Sep 20, 2019·The Plant Journal : for Cell and Molecular Biology·Eun-Cheon LimKyuha Choi
Sep 23, 2020·Antioxidants·Razan MuhtadiHarry Scherthan

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Methods Mentioned

BETA
flow cytometry
PCR
restriction digest
random spore

Software Mentioned

NEBuilder
Adobe Photoshop CC
ImageStreamX
INSPIRE
AxioVision 40
Excel
IDEAS
ImageStream

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