Oct 31, 2018

Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis

BioRxiv : the Preprint Server for Biology
Dmitriy LiAlexander Lorenz

Abstract

Schizosaccharomyces pombe , also known as fission yeast, is an established model for studying chromosome biological processes. Over the years research employing fission yeast has made important contributions to our knowledge about chromosome segregation during meiosis, as well as meiotic recombination and its regulation. Quantification of meiotic recombination frequency is not a straightforward undertaking, either requiring viable progeny for a genetic plating assay, or relying on laborious Southern blot analysis of recombination intermediates. Neither of these methods lends itself to high-throughput screens to identify novel meiotic factors. Here, we establish visual assays novel to Sz. pombe for characterizing chromosome segregation and meiotic recombination phenotypes. Genes expressing red, yellow, and/or cyan fluorophores from spore-autonomous promoters have been integrated into the fission yeast genomes, either close to the centromere of chromosome I to monitor chromosome segregation, or on the arm of chromosome III to form a genetic interval at which recombination frequency can be determined. The visual recombination assay allows straightforward and immediate assessment of the genetic outcome of a single meiosis by epi-fl...Continue Reading

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Mentioned in this Paper

Meiotic Cell Cycle
Flow Cytometry
Research
Genome
Genes
Regulation of Biological Process
High Throughput Screening
Spores, Bacterial
Recombination, Genetic
Reproduction Spores

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