PMID: 15332596Aug 31, 2004Paper

Immobilisation of catalase on the surface of biodegradable starch-based polymers as a way to change its surface characteristics

Journal of Materials Science. Materials in Medicine
S A Costa, R L Reis

Abstract

In this study, a specific enzyme catalase was immobilised onto the surface of two different biodegradable materials, starch cellulose acetate (SCA) and starch polycrapolactone (SPCL) blends. This immobilisation was achieved by several different routes, mainly by covalent binding and an adsorption method using as activation agents epichlorohydrin, cyanogen bromide (CNBr), and aminopropyltriethoxysilane. The effect of the coupling pH of the enzyme-support reaction was determined in terms of activity recovery (%). The catalase immobilised on SCA showed higher activity recovery (%) for all the methods used as compared with results obtained with SPCL. The immobilisation process using epichlorohydrin as an activation agent and polyethylenimine as a spacer-arm enhanced the stability and the half-lives at pH 7.0, 30 degrees C, for immobilised catalase on both SCA and SPCL. The half-lives were respectively, 1162 and 870 h compared with other treatments and free enzyme (480 h). The free glycerol present in the immobilisation medium was also a factor that contributed toward the better performance regarding the long-term stability at 30 degrees C and neutral pH. The extension of the morphological modifications on the surface of the materia...Continue Reading

Citations

Feb 3, 2015·Critical Reviews in Biotechnology·Valeria E BosioGuillermo R Castro
Nov 20, 2014·Journal of Biomedical Materials Research. Part a·Soheila Ali Akbari GhavimiNoor Azuan Abu Osman
Nov 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Bo ChenRichard A Gross

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