Immobilization and stabilization of xylanase by multipoint covalent attachment on agarose and on chitosan supports

Applied Biochemistry and Biotechnology
Anny ManrichRaquel L C Giordano

Abstract

Xylanases have important applications in industry. Immobilization and stabilization of enzymes may allow their reuse in many cycles of the reaction, decreasing the process costs. This work proposes the use of a rational approach to obtain immobilized commercial xylanase biocatalysts with optimized features. Xylanase NS50014 from Novozymes was characterized and immobilized on glyoxyl-agarose, agarose-glutaraldehyde, and agarose-amino-epoxy support and on differently activated chitosan supports: glutaraldehyde-chitosan, glyoxyl-chitosan, and epoxy-chitosan. Two different chitosan matrices were tested. The best chitosan derivative was epoxy-chitosan-xylanase, which presented 100% of immobilization yield and 64% of recovered activity. No significant increase on the thermal stability was observed for all the chitosan-enzyme derivatives. Immobilization on glyoxyl-agarose showed low yield immobilization and stabilization degrees of the obtained derivative. The low concentration of lysine groups in the enzyme molecule could explain these poor results. The protein was then chemically modified with ethylenediamine and immobilized on glyoxyl-agarose. The new enzyme derivatives were 40-fold more stable than the soluble, aminated, and dialy...Continue Reading

References

Nov 1, 1992·Enzyme and Microbial Technology·S S Wong, L J Wong
Sep 11, 2001·Applied Microbiology and Biotechnology·Q K BegG S Hoondal
Apr 5, 2003·Biotechnology Progress·Paulo W TardioliJosé M Guisán
Jan 20, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·J BergerR Gurny
Jan 18, 2005·Journal of Biotechnology·Fernando López-GallegoRoberto Fernández-Lafuente
Oct 20, 2006·Trends in Biotechnology·B Hahn-HägerdalG Zacchi
Dec 25, 2007·Biochimica Et Biophysica Acta·Shweta Shah, Munishwar N Gupta
Jan 12, 2008·International Journal of Biological Macromolecules·Anny ManrichRaquel L C Giordano
Jul 18, 2008·Biomacromolecules·Wellington S AdrianoRaquel L C Giordano

❮ Previous
Next ❯

Citations

Oct 1, 2013·Applied Biochemistry and Biotechnology·Semra Yilmazer Keskin, Can Serkan Keskin
Feb 3, 2012·Advanced Materials·Pamela Torres-SalasFrancisco J Plou
Dec 21, 2011·Journal of Biomaterials Science. Polymer Edition·E VardarN Hasirci
Sep 21, 2013·Applied Biochemistry and Biotechnology· Preety, Vinita Hooda
May 22, 2021·Applied Biochemistry and Biotechnology·Sabrina Ait BrahamRoberto Fernandez-Lafuente
Aug 30, 2021·Biotechnology Advances·Rafael C RodriguesRoberto Fernandez-Lafuente

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.