Immobilization of a recombinant esterase from Lactobacillus plantarum on polypropylene Accurel MP1000

Applied Biochemistry and Biotechnology
Deise Juliana KollingAna Carolina Maisonnave Arisi

Abstract

A recombinant esterase from Lactobacillus plantarum was immobilized on hydrophobic support polypropylene Accurel MP1000 by adsorption. Adsorption efficiency was 83%, and the immobilized protein was 12.4 mg/g of support. Esterase activity was determined using p-nitrophenyl butyrate as substrate, and highest activities were observed at 50 °C for immobilized enzyme and 30 °C for free enzyme extract. Concerning thermal stability, after enzyme incubation at 80 °C for 30 min, immobilized and free enzyme retained 91% and 56% of initial activity, respectively. Immobilized enzyme presented lower V(max) and higher K(m) than free enzyme. Protein was not released from the support, and esterase activity increased after 3 cycles of reuse.

References

May 3, 2000·Journal of Applied Microbiology·K M FensterJ L Steele
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Nov 6, 2004·Bio Systems·Songjun LiBailing Liu
Feb 16, 2005·Applied Microbiology and Biotechnology·Namita GuptaRani Gupta
Sep 21, 2007·Bioprocess and Biosystems Engineering·Annapurna KumariRintu Banerjee
Mar 11, 2008·Applied Biochemistry and Biotechnology·Wazé Aimée Mireille AllouePhilippe Thonart
Jun 11, 2008·Applied Biochemistry and Biotechnology·Sebastián TorresGuillermo R Castro
Aug 8, 2008·Colloids and Surfaces. B, Biointerfaces·Maha Karra-ChâabouniYoussef Gargouri
Aug 16, 2008·Journal of Industrial Microbiology & Biotechnology·M Guadalupe Sánchez-OteroRosa María Oliart-Ros
Nov 28, 2008·Applied Biochemistry and Biotechnology·Aline G CunhaDenise M G Freire
Dec 25, 2009·Molecular Biotechnology·Fábio Cristiano Angonesi BrodAna Carolina Maisonnave Arisi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.