Immobilization of aptamer-modified gold nanoparticles on BiOCl nanosheets: Tunable peroxidase-like activity by protein recognition

Biosensors & Bioelectronics
Chia-Lun HsuHuan-Tsung Chang

Abstract

A self-assembled nanocomposite is prepared from an aqueous mixture of aptamer-modified gold nanoparticles (Apt-Au NPs), bismuth ions and chloride ions. The Apt-Au NPs are immobilized on bismuth oxychloride (BiOCl) nanosheets in situ to form Apt-Au NPs/BiOCl nanocomposites. The as-prepared nanocomposites exhibit high peroxidase-like activity for the catalytic conversion of Amplex Red (AR) to fluorescent resorufin in the presence of H2O2. The catalytic activity of Apt-Au NPs/BiOCl nanocomposites is at least 90-fold higher than that of Apt-Au NPs or BiOCl nanosheets, revealing synergistic effects on their activity. The catalytic activity of Apt-Au NPs/BiOCl nanocomposites is suppressed by vascular endothelial growth factor-A165 (VEGF-A165) molecules that specifically interact with the aptamer units (Del-5-1 and v7t-1) on the nanocomposite surface. The AR/H2O2-Apt-Au NPs/BiOCl nanocomposites probe shows high selectivity (>1000-fold over other proteins) and sensitivity (detection limit ~0.5nM) for the detection of VEGF-A165. Furthermore, the probe is employed for the detection of VEGF isoforms and for the study of interactions between VEGF and VEGF receptors. The practicality of this simple, rapid, cost-effective probe is validated ...Continue Reading

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Citations

Jan 5, 2019·Advanced Pharmaceutical Bulletin·Fatemeh AkbarzadehDavood Rezazadeh
Jun 9, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Jinyi Zhang, Juewen Liu
Jul 16, 2019·Environmental Science and Pollution Research International·Hujiabudula MaimaitiziAbulikemu Abulizi
Nov 24, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lu TianRun Zhang
Jul 14, 2020·Journal of Hazardous Materials·Binesh UnnikrishnanChih-Ching Huang
Apr 17, 2021·ACS Biomaterials Science & Engineering·Niranjana Jaya PrakashBalasubramanian Kandasubramanian
Jun 23, 2020·ACS Omega·Rawdha DekhiliJolanda Spadavecchia
Jan 18, 2018·ACS Applied Materials & Interfaces·Ruibo ZhongLi Li
Sep 10, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qingqing WangZhiyong Tang

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