Immobilization of Aspergillus niger cellulase on multiwall carbon nanotubes for cellulose hydrolysis

Bioresource Technology
Razi Ahmad, S K Khare

Abstract

In present study, Aspergillus niger cellulase was immobilized onto functionalized multiwalled carbon nanotubes (MWCNTs) via carbodiimide coupling. MWCNTs offer unique advantages including enhanced electronics properties, a large edge to basal plane ratio, rapid electrode kinetics and it's possess higher tensile strength properties due to their structural arrangements. The immobilization was confirmed by FTIR (Fourier transform infrared spectroscopy) and SEM (scanning electron microscope). The bionanoconjugates prepared under optimized condition retained 85% activity with improved pH and thermal stability. The t1/2 of immobilized cellulase at 70 °C was four fold higher than free enzyme. The Km value indicates that affinity of bionanoconjugates towards substrate has increased by two times. The preparation could be reused ten times without much loss in enzyme activity. The enhanced catalytic efficiency, stability and reusability makes it useful for efficient cellulose hydrolysis.

Citations

Apr 24, 2019·Analytical and Bioanalytical Chemistry·Bin ZouJiaojiao Xia
Nov 25, 2020·International Journal of Biological Macromolecules·Zahra AshkanAmin Tashakor
Mar 14, 2021·Carbohydrate Polymers·Bin HeSufeng Zhang
Sep 23, 2020·International Journal of Biological Macromolecules·Syeda Warisul FatimaSunil K Khare
Jun 1, 2021·International Journal of Biological Macromolecules·K Narayanan RajnishEthiraj Selvarajan
May 25, 2021·Frontiers in Microbiology·Shubhrima GhoshShakilur Rahman
Jul 3, 2021·Frontiers in Genetics·Md ZeyaullahRazi Ahmad
Aug 30, 2021·Environmental Science and Pollution Research International·Basem E KeshtaAbeer A Khamis
Sep 8, 2021·Journal of Food Biochemistry·Chong-Boon Ong, Mohamad Suffian Mohamad Annuar

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