Immobilization of Aspergillus niger lipase on chitosan-coated magnetic nanoparticles using two covalent-binding methods

Bioprocess and Biosystems Engineering
Yolanda OsunaAnna Ilyina

Abstract

Aspergillus niger lipase immobilization by covalent binding on chitosan-coated magnetic nanoparticles (CMNP), obtained by one-step co-precipitation, was studied. Hydroxyl and amino groups of support were activated using glycidol and glutaraldehyde, respectively. Fourier transform infrared spectrometry, high-resolution transmission electron microscopy and thermogravimetric analysis confirmed reaction of these coupling agents with the enzyme and achievement of a successful immobilization. The derivatives showed activities of 309.5 ± 2.0 and 266.2 ± 2.8 U (g support)(-1) for the CMNP treated with glutaraldehyde and with glycidol, respectively. Immobilization enhanced the enzyme stability against changes of pH and temperature, compared to free lipase. Furthermore, the kinetic parameters K m and V max were determined for the free and immobilized enzyme. K m value quantified for enzyme immobilized by means of glutaraldehyde was 1.7 times lowers than for free lipase. High storage stability during 50 days was observed in the immobilized derivatives. Finally, immobilized derivatives retained above 80% of their initial activity after 15 hydrolytic cycles. The immobilized enzyme can be applied in various biotechnological processes involvi...Continue Reading

References

Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Apr 10, 2007·Applied Biochemistry and Biotechnology·Zhen-Xing TangLu-E Shi
Jan 20, 1990·Biotechnology and Bioengineering·J F ShawY J Wang
Feb 2, 2010·Applied Biochemistry and Biotechnology·Anny ManrichRaquel Lima Camargo Giordano
Feb 16, 2010·Biotechnology Letters·Jun HuangLiyun Ding
Jun 29, 2010·Biotechnology Advances·Tianwei TanFang Wang
Feb 25, 2011·Bioprocess and Biosystems Engineering·Nevena Z PrlainovićDejan I Bezbradica

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Citations

Oct 17, 2015·Biotechnology Letters·Hamideh VaghariShahin Nasiri
Aug 17, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Maria Elisa Melo Branco de AraújoPatrícia de Oliveira Carvalho
Dec 30, 2016·Preparative Biochemistry & Biotechnology·Sócrates Palacios-PonceAnna Ilyina
Aug 2, 2017·Applied Biochemistry and Biotechnology·Sinem Diken GürNilüfer Aksöz
Aug 21, 2019·International Journal of Molecular Sciences·Rodolpho R C MonteiroRoberto Fernandez-Lafuente
Dec 15, 2020·Preparative Biochemistry & Biotechnology·Mariana Vendrasco TacinValéria de Carvalho Santos-Ebinuma
Mar 6, 2021·International Journal of Biological Macromolecules·F Rafiee, M Rezaee
Apr 18, 2021·International Journal of Biological Macromolecules·Yale Luck NunesJosé C S Dos Santos
Oct 12, 2021·Journal of Biotechnology·Daniel Alberto SánchezMaría Lujan Ferreira

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