Immobilization of cellulase on a silica gel substrate modified using a 3-APTES self-assembled monolayer

SpringerPlus
Dezhi ZhangJames Palmer

Abstract

Cellulase was immobilized onto silica gel surfaces pretreated with (3-aminopropyl) triethoxy-silane (3-APTES), and glutaraldehyde (GA) was used as a cross-linker. A carboxymethyl cellulose sodium salt (CMC) solution was used for activity experiments. Protein assay was performed to determine the mass immobilized and compare with free enzyme. Cellulase was successfully demonstrated to be immobilized on the modified silica gel surface, and no detectable amount of enzyme was stripped off during the hydrolysis of the CMC solution. The specific activity of the immobilized cellulase is 7 ± 2 % compared to the similar amount of free cellulase. Significant activity over multiple reuses was observed. The seventh batch achieved 82 % activity of the initial batch, and the fifteenth batch retained 31 %. It was observed that the immobilized cellulase retained 48 % of its initial activity after 4 days, and 22 % even after 14 days.

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Citations

Jun 21, 2018·Chemical Society Reviews·Joshua BrittonGregory A Weiss
Feb 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ilayda BerktasBurcu Saner Okan
Sep 16, 2020·Nanomaterials·Aniello CostantiniFilomena Sannino
Feb 9, 2021·ACS Biomaterials Science & Engineering·Jyotsana PriyadarshaniSuman Chakraborty
Apr 3, 2018·ACS Omega·Md Amzad HossainLee D Wilson
Feb 6, 2020·ACS Applied Materials & Interfaces·Shuhao LiuMustafa Akbulut

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Methods Mentioned

BETA
protein assay
Infrared Spectroscopy
protein

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