PMID: 39611Sep 12, 1979

Immobilization of Escherichia coli cells containing aspartase activity with kappa-carrageenan. Enzymic properties and application for L-aspartic acid production

Biochimica Et Biophysica Acta
T SatoI Chibata

Abstract

Whole cells of Escherichia coli having high aspartase (L-asparate ammonialyase, EC 4.3.1.1) activity were immobilized by entrapping into a kappa-carrageenan gel. The obtained immobilized cells were treated with glutaraldehyde or with glutaraldehyde and hexamethylenediamine. The enzymic properties of three immobilized cell preparations were investigated, and compared with those of the soluble aspartate. The optimum pH of the aspartase reaction was 9.0 for the three immobilized cell preparations and 9.5 for the soluble enzyme. The optimum temperature for three immobilized cell preparations was 5--10 degrees C higher than that for the soluble enzyme. The apparent Km values of immobilized cell preparations were about five times higher than that of the soluble enzyme. The heat stability of intact cells was increased by immobilization. The operational stability of the immobilized cell columns was higher at pH 8.5 than at optimum pH of the aspartase reaction. From the column effluents, L-aspartic acid was obtained in a good yield.

Citations

Jan 1, 1983·Folia Microbiologica·V Vojtísek, V Jirků
Oct 15, 2013·Applied and Environmental Microbiology·Joseph P ParkHaeshin Lee
Jan 1, 1988·Annals of the New York Academy of Sciences·S Shimizu, H Yamada
Oct 5, 1990·Biotechnology and Bioengineering·P G IbbotsonM S Otterburn
Jul 1, 1986·Biotechnology and Bioengineering·V VojtsekJ Netnral
Oct 1, 1982·Biotechnology and Bioengineering·H S Kim, D D Ryu
Aug 9, 2019·Evolution; International Journal of Organic Evolution·Sanne WesthoffDaniel E Rozen
Jan 8, 2020·Critical Reviews in Biochemistry and Molecular Biology·Ronald E Viola
Jan 1, 1994·Critical Reviews in Biotechnology·S Norton, J C Vuillemard
Feb 26, 2013·Chemical Society Reviews·Robert DiCosimoGregory Bohlmann
Dec 19, 2016·Applied Biochemistry and Biotechnology·Arti T PatelDarshan H Patel

Related Concepts

Ammonia-Lyases
Aspartate Ammonia-Lyase
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
lambda-Carrageenan
Alkalescens-Dispar Group
Hydrogen-Ion Concentration

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