Immobilization of fungal beta-glucosidase on silica gel and kaolin carriers

Applied Biochemistry and Biotechnology
Hakob K KaragulyanStephen R Decker

Abstract

Beta-glucosidase is a key enzyme in the hydrolysis of cellulose for producing feedstock glucose for various industrial processes. Reuse of enzyme through immobilization can significantly improve the economic characteristics of the process. Immobilization of the fungal beta-glucosidase by covalent binding and physical adsorption on silica gel and kaolin was conducted for consequent application of these procedures in large-scale industrial processes. Different immobilization parameters (incubation time, ionic strength, pH, enzyme/support ratio, glutaric aldehyde concentration, etc.) were evaluated for their effect on the thermal stability of the immobilized enzyme. It was shown that the immobilized enzyme activity is stable at 50 degrees C over 8 days. It has also been shown that in the case of immobilization on kaolin, approximately 95% of the initial enzyme was immobilized onto support, and loss of activity was not observed. However, covalent binding of the enzyme to silica gel brings significant loss of enzyme activity, and only 35% of activity was preserved. In the case of physical adsorption on kaolin, gradual desorption of enzyme takes place. To prevent this process, we have carried out chemical modification of the protein....Continue Reading

References

Apr 20, 2002·Applied Biochemistry and Biotechnology·L P CalsavaraG M Zanin
Jan 25, 2005·Bioscience, Biotechnology, and Biochemistry·Hidetaka NagatomoTakanao Matsui
Oct 20, 2005·Journal of Bioscience and Bioengineering·Sosaku IchikawaSukekuni Mukataka

Citations

Dec 22, 2009·Preparative Biochemistry & Biotechnology·Fang WangZhi-Min He
Jan 10, 2016·Bioprocess and Biosystems Engineering·Nataša Ž ŠekuljicaZorica D Knežević-Jugović
Sep 15, 2011·Enzyme Research·Joelise de Alencar FigueiraPedro Fernandes
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natália G GraebinRafael C Rodrigues
Jan 1, 2013·3 Biotech·Sumitra DattaYamuna Rani Sriramulu Rajaram
Mar 31, 2019·Bioprocess and Biosystems Engineering·Janaina de Souza LimaDébora de Oliveira

Related Concepts

Adsorption
Emulsion beta-D-Glucosidase
Enzyme Activation
Enzyme Stability
Yeast Proteins
Kaolinite
Quso G-32
Beta-Glucosidase
Cellulose
Fungi

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