Immobilization of gene vectors on polyurethane surfaces using a monoclonal antibody for localized gene delivery

The Journal of Gene Medicine
Lin MeiRobert J Levy

Abstract

Conventional strategies of gene therapy using viral vectors result in suboptimal localization and potentially dangerous distal spread of vector. We hypothesized that localized delivery of adenoviral gene vectors could be achieved from a polyurethane (PU) film through a mechanism involving anti-viral antibody tethering. PU films were formulated with a collagen coating. Anti-adenoviral monoclonal antibodies were covalently bound to the collagen surface. These antibodies enabled tethering of replication-defective adenoviruses [Ad-GFP (encoding green fluorescent protein)] through highly specific antigen-antibody affinity. The binding stability and in vitro delivery of virus bound on PU films were investigated. Cell culture studies with rat arterial smooth muscle cells (A10) assessed transduction on or near the PU matrix. In vivo experiments with collagen-coated PU films investigated atrial epicardial implant and subdermal implant models in Yorkshire swine. We report for the first time successful PU film-based gene delivery using antibody-tethered adenovirus encoding the green fluorescent protein (GFP), demonstrating efficient and highly localized gene delivery to arterial smooth muscle cells in cell culture and pig implant. In comp...Continue Reading

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Citations

Feb 20, 2008·Journal of Materials Science. Materials in Medicine·Timothy Douglas, Håvard J Haugen
Jun 2, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jae-Hyung JangLonnie D Shea
Jan 7, 2011·Trends in Biotechnology·Chung-Huei K Wang, Suzie H Pun
Mar 6, 2010·International Journal of Cardiology·Robert W BoldermanJos G Maessen
Jun 23, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·I-Chien LiaoKam W Leong
Jul 12, 2008·The Journal of Gene Medicine·Wei-Wen HuPaul H Krebsbach
Apr 23, 2013·Biomaterials·R M BoehlerL D Shea
Dec 27, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guilei MaCunxian Song
Nov 17, 2020·Advanced Healthcare Materials·Yi WangDavid R Nisbet
Jul 4, 2012·The Journal of Physical Chemistry. B·Anna KovtunMatthias Epple

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