PMID: 2105696Jan 1, 1990Paper

Immobilization of glucose oxidase and peroxidase and their application in flow-injection analysis for glucose in serum

Applied Biochemistry and Biotechnology
J A GuoG X Li

Abstract

Glucose oxidase (GOD) and Horseradish peroxidase (HRP) were covalently coupled to alkylamine controlled pore glass by means of glutaraldehyde. About 700-800 U/g of immobilized GOD and 300-400 U/g of immobilized HRP were obtained. Some factors of affecting enzyme immobilization were discussed. The immobilized enzymes were packed into a plastic tube and used in flow-injection analysis (FIA) for glucose in serum. A good linearity range was observed for this immobilized enzyme system at 20 mg/mL to 1000 mg/dL D-glucose, the recovery was 95.4-103.5%, the within-batch imprecision was 0.8-2.2%, and the between-batch imprecision was 2.2-4.2%. More than 100 samples were measured within an hour. One enzyme column with five units of immobilized GOD and HRP, applied for 50 assays/d, has been used for more than 2 mo.

References

Sep 22, 1971·Biochimica Et Biophysica Acta·P J RobinsonM D Lilly
Sep 1, 1982·Applied Biochemistry and Biotechnology·L GaoxiangZ Shuzheng

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Citations

Jul 5, 1996·Biotechnology and Bioengineering·K TatsumiH Ichikawa
Jul 11, 2014·Experimental and Therapeutic Medicine·Linlin TangShiwei Duan

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