Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle

Biochemical and Biophysical Research Communications
Yong Taik LimBong Hyun Chung

Abstract

We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent mole...Continue Reading

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Citations

Dec 25, 2007·Analytical and Bioanalytical Chemistry·Pedro BaptistaRicardo Franco
Nov 5, 2010·Analytical and Bioanalytical Chemistry·Dario Lombardi, Petra S Dittrich
Aug 30, 2008·Journal of Biomaterials Science. Polymer Edition·Gwang-Chol KimRen-Xi Zhuo
Oct 25, 2007·Chembiochem : a European Journal of Chemical Biology·Yong Taik LimBong Hyun Chung
Dec 6, 2008·Small·Yong Taik LimBong Hyun Chung
Feb 6, 2009·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Eleonora V ShtykovaLyudmila M Bronstein
Jan 12, 2011·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Lyudmila M BronsteinDmitri I Svergun
Feb 7, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Angela M BudginLyudmila M Bronstein
Dec 14, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Samuel H GageLyudmila M Bronstein

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