Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

Enzyme and Microbial Technology
Evelin A ManoelR Fernández-Lafuente

Abstract

The lipases from Thermomyces lanuginosus and Pseudomonas cepacia have been immobilized on octyl and cyanogen bromide (CNBr) agarose beads. The immobilization on octyl-agarose is slowed with increasing ionic strength, while the immobilization on CNBr is not significantly affected by the ionic strength. The inhibition of the immobilized preparations with diethyl p-nitrophenylphosphate (D-pNPP) was analyzed. The inhibition was more rapid using octyl-lipase preparations than using covalent preparations, and the covalent preparations were much more sensitive to the reaction medium. The addition of detergent increased the inhibition rate of the covalent preparation while an increase on the ionic strength produced a slowdown of the inhibition rate by D-pNPP for both lipases. The effect of the medium on the activity versus fully soluble substrate (methyl mandelate) was in the same direction. The octyl preparations presented a slight decrease in activity when comparing the results using different concentrations of sodium phosphate buffer (between 0.025 and 1M), while the CNBr preparations suffered drastic drops in its activity at high ionic strength. The results confirm that the lipases immobilized on octyl agarose presented their open ...Continue Reading

References

Nov 1, 1993·Trends in Biotechnology·E Katchalski-Katzir
Aug 1, 1996·Current Opinion in Structural Biology·C CambillauC Martinez
Aug 28, 1998·Chemistry and Physics of Lipids·R Fernandez-LafuenteJ M Guisán
Sep 23, 1998·Trends in Biotechnology·K E Jaeger, M T Reetz
Nov 26, 1999·Biochimica Et Biophysica Acta·M Cygler, J D Schrag
Sep 27, 2002·Current Opinion in Biotechnology·Karl-Erich Jaeger, Thorsten Eggert
Feb 14, 2003·Biotechnology and Bioengineering·Gloria Fernández-LorenteRoberto Fernández-Lafuente
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Mar 24, 2004·Trends in Biotechnology·Oscar Pàmies, Jan-E Bäckvall
Jul 6, 2004·Journal of Chromatography. a·Jose M PalomoRoberto Fernandez-Lafuente
Feb 11, 1983·Science·A M Klibanov
Apr 25, 1994·Biotechnology and Bioengineering·J A Bosley, J C Clayton
Jul 11, 2009·Biotechnology Letters·Dean Brady, Justin Jordaan
Oct 13, 2012·Chemical Society Reviews·Rafael C RodriguesRoberto Fernández-Lafuente
Feb 14, 2013·Chemical Society Reviews·Patrick Adlercreutz
May 20, 2014·Enzyme and Microbial Technology·Jose C S dos SantosRoberto Fernandez-Lafuente

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Jun 1, 2016·Enzyme and Microbial Technology·María VillalbaCristina Otero
May 12, 2016·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Nazzoly RuedaRoberto Fernandez-Lafuente
May 20, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nazzoly RuedaRoberto Fernandez-Lafuente
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Oct 23, 2016·Colloids and Surfaces. B, Biointerfaces·Marco MuthKlaus Schnitzlein
Nov 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paolo ZuccaEnrico Sanjust
Dec 3, 2016·Applied Biochemistry and Biotechnology·Shamraja S Nadar, Virendra K Rathod
Mar 30, 2017·Applied Biochemistry and Biotechnology·Xiaomei WanYi Hu
Nov 17, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francielle Batista da SilvaRosana Maria Nascimento Assunção
Sep 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marita G PereiraMaria de Lourdes T M Polizeli
Mar 28, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lionete Nunes de LimaRaquel de Lima Camargo Giordano
Mar 15, 2018·Biotechnology Progress·Maísa Pessoa PinheiroLuciana R B Gonçalves
Oct 12, 2017·Applied Biochemistry and Biotechnology·Ulisses M F de OliveiraLuciana R B Gonçalves
Jun 28, 2018·Physical Chemistry Chemical Physics : PCCP·Pegah S Nabavi ZadehBjörn Åkerman
Feb 8, 2019·Applied Biochemistry and Biotechnology·Daniela V RossetSérgio L Jahn
Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Evelin Sánta-BellLászló Poppe
Jul 16, 2019·NPJ Science of Food·Stephanie M Andler, Julie M Goddard
Aug 8, 2020·Journal of Oleo Science·Fenghuan ZhaoNanjing Zhong
Mar 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniela F M TuratiEleonora C Carmona
Sep 28, 2017·Bioengineering·Rajeev Ravindran, Amit K Jaiswal
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing SunQipeng Yuan
Nov 7, 2019·Biomolecules·Karina D Garcia-OrozcoRogerio R Sotelo-Mundo
Jul 1, 2020·Bioprocess and Biosystems Engineering·Janaina Pires BorgesJosé Manuel Guisan
Jul 1, 2017·Applied Biochemistry and Biotechnology·Horacio L BonazzaEnrique J Mammarella
Dec 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valeria CalifanoLuciano Vicari
Jul 30, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shan ZhangFenghong Huang
Sep 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lívia T de A SouzaBenevides C Pessela
Jun 19, 2018·Applied Biochemistry and Biotechnology·Radosław DrozdKarol Fijałkowski
Nov 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Malena Martínez PérezMaria de Lourdes Teixeira de Moraes Polizeli

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