Immobilization of lysozyme on bacterial cellulose nanofibers: Characteristics, antimicrobial activity and morphological properties

International Journal of Biological Macromolecules
Pashew BayazidiAsghar Khosrowshahi Asl

Abstract

The aim of this study was to immobilize of lysozyme onto bacterial cellulose nanofibers (BCNF) using physical absorption method. Antimicrobial activity and other properties of immobilized lysozyme and also morphological characteristics of BCNF were studied. Activity of lysozyme was decreased about 12% after immobilization. The lysozyme concentration of 1mg/ml was chosen for next studies on immobilized enzyme. The optimum pH and temperature were recorded as 7 and 45°C, respectively for immobilized enzyme. Storage stability was increased and immobilized lysozyme exhibited more than 70% of its initial activity after 9 cycles. Kinetic parameters of Km and Vmax were 31.18μg/ml and 112.35 ΔOD/min, respectively for immobilized enzyme. Antimicrobial activity of lysozyme against Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Yersinia entrocolitica, Aspergillus niger and Saccharomyces serviseae was increased after immobilization. The XRD analyses showed a decrease in diffraction intensities and crystallinity index of BCNF after immobilization of lysozyme. According to the results of scanning electron microscopy (SEM), the diameter of nanofibers was increased by lysozyme incorporation. This study demonstrates the potenti...Continue Reading

Citations

Apr 24, 2020·Critical Reviews in Food Science and Nutrition·Hamed Bagheri DarvishLeonard Williams
May 1, 2020·Bioprocess and Biosystems Engineering·Wenli YangYuanyuan Yu
Jun 27, 2020·Critical Reviews in Food Science and Nutrition·Hadi AlmasiAyda Saleh
Sep 25, 2019·Nanomaterials·Selestina Gorgieva, Janja Trček
Sep 24, 2020·International Journal of Environmental Research and Public Health·Ruth NaomiMh Busra Fauzi
Oct 22, 2020·Nanomaterials·Francisco A G S SilvaFátima Poças
Dec 3, 2020·Polymers·Kaja KupnikMaja Leitgeb
Feb 16, 2021·International Journal of Nanomedicine·Carolina Maria Costa de Oliveira SouzaKatherine Athayde Teixeira de Carvalho
Feb 23, 2020·International Journal of Biological Macromolecules·Sharanya SarkarKrishna Mohan Poluri
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Mengqin YuanHongbing Deng
Feb 26, 2021·Biotechnology Advances·Hao DongPing Wang
Mar 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cornelia Vasile, Mihaela Baican
Mar 30, 2021·Biosensors & Bioelectronics·Madalina Elena CulicaSergiu Coseri
May 2, 2021·Colloids and Surfaces. B, Biointerfaces·Sahar ZahirinejadFabio Vianello
May 23, 2021·International Journal of Biological Macromolecules·Bo Kyeong YoonJoshua A Jackman
Jul 4, 2021·International Journal of Biological Macromolecules·Annie A CerónSirlene M Costa
Jul 30, 2021·Bioresource Technology·R ReshmyRaveendran Sindhu
Aug 5, 2020·International Journal of Biological Macromolecules·Isabela de Andrade Arruda FernandesCharles Windson Isidoro Haminiuk

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Sai LiYinping Zhong
© 2022 Meta ULC. All rights reserved