Abstract
Background/aims The critical part of a bio-artificial liver device is establishment of a bioreactor filled with liver cells. However, it is still unclear how to maintain benign cell function while achieving the sufficient cell quantity. In the current study, we aim to establish a novel carrier for the culture of HepG2 cells, a liver cell line, by modifying polypropylene nonwoven fabrics with native type I collagen. Methods "Piranha" solution, KH-550 and glutaraldehyde subsequently were used to bridge native type I collagen and polypropylene nonwoven fabrics. The type I collagen-coupled polypropylene nonwoven fabric was characterized by XPS, SEM, ATR-FTIR and water contact angle measurement. Furthermore, the biocompatibility between HepG2 cells and fiber film is evaluated by the ability of cell proliferation, albumin secretion, as well as urea synthesis. Results The coating of collagen onto polypropylene fabrics was more efficient using the chemical covalent binding method than direct immersion, which was validated by the presence of collagen-related elements and chemical bond. The adding of collagen in polypropylene fabrics promoted hydrophilicity and HepG2 cell adherence. Additionally, enhanced cell proliferation, increased al...Continue Reading
References
Sep 15, 2000·The Journal of Biological Chemistry·Y XuM Höök
Jun 29, 2004·Proteomics·Taewoon ChaXiao-Yang Zhu
Dec 15, 2006·World Journal of Gastroenterology : WJG·Yasushi KawazoeTakashi Kanematsu
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Chen WuYaoting Yu
Aug 5, 2008·Colloids and Surfaces. B, Biointerfaces·Yang YaoXi-Zeng Feng
Nov 20, 2009·Gut·B CarpentierC Legallais
Aug 11, 2010·Journal of Biotechnology·Y ParkW S Hu
Oct 5, 2010·Cell Transplantation·Li-Yi SunTzyy-Wen Chiou
Nov 26, 2010·Journal of Biomedical Materials Research. Part a·Ting Ting LauDong-An Wang
Dec 25, 2010·Connective Tissue Research·J P R O OrgelO Antipova
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·François BerthiaumeMartin L Yarmush
Dec 4, 2012·Colloids and Surfaces. B, Biointerfaces·Fupo HeJiandong Ye
Feb 1, 2014·Journal of Biotechnology·Yonsil ParkCatherine M Verfaillie
Mar 19, 2014·Biopolymers·Sayani Chattopadhyay, Ronald T Raines
Mar 22, 2014·Biotechnology Letters·Guang-Zhen JinHae-Won Kim
Apr 11, 2014·Bioengineered·John A M RamshawGeoff J Dumsday
Jul 24, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Helena P FelgueirasVéronique Migonney
Jan 8, 2015·Journal of Hepato-biliary-pancreatic Sciences·Keiichi FujiwaraOsamu Yokosuka
Jan 16, 2016·Cell Research·Xiao-Lei ShiLijian Hui
Jan 29, 2016·Medicine·Yi ShenGang Qin
May 22, 2016·Materials Science & Engineering. C, Materials for Biological Applications·A R SadeghiA Mahdizadeh
Jun 15, 2016·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Jia-Jia ChenLan-Juan Li
Jun 25, 2016·Critical Care Clinics·Constantine J Karvellas, Ram M Subramanian
Sep 20, 2016·Stem Cells·Clara T NicolasScott L Nyberg
Feb 4, 2016·Polymers·Chanjuan Dong, Yonggang Lv