Immobilization of native type I collagen on polypropylene fabrics as a substrate for HepG2 cell culture

Journal of Biomaterials Applications
Gongze PengYi Gao

Abstract

Background/aims The critical part of a bio-artificial liver device is establishment of a bioreactor filled with liver cells. However, it is still unclear how to maintain benign cell function while achieving the sufficient cell quantity. In the current study, we aim to establish a novel carrier for the culture of HepG2 cells, a liver cell line, by modifying polypropylene nonwoven fabrics with native type I collagen. Methods "Piranha" solution, KH-550 and glutaraldehyde subsequently were used to bridge native type I collagen and polypropylene nonwoven fabrics. The type I collagen-coupled polypropylene nonwoven fabric was characterized by XPS, SEM, ATR-FTIR and water contact angle measurement. Furthermore, the biocompatibility between HepG2 cells and fiber film is evaluated by the ability of cell proliferation, albumin secretion, as well as urea synthesis. Results The coating of collagen onto polypropylene fabrics was more efficient using the chemical covalent binding method than direct immersion, which was validated by the presence of collagen-related elements and chemical bond. The adding of collagen in polypropylene fabrics promoted hydrophilicity and HepG2 cell adherence. Additionally, enhanced cell proliferation, increased al...Continue Reading

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Citations

Feb 23, 2019·Journal of Dental Research·C M HsuH H Huang
Mar 13, 2020·Journal of Biomaterials Science. Polymer Edition·Chaochen ZhaoYi Gao
Jun 25, 2020·Journal of Biomaterials Applications·Di MengGuifeng Zhang

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Methods Mentioned

BETA
chemical exchange
X-ray
infrared
scanning electron microscopy
ELISA
Fluorescence

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Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.