Immobilization of pancreatic lipase on chitin and chitosan

Preparative Biochemistry & Biotechnology
Ali KilinçAzmi Telefoncu

Abstract

In this study, porcine pancreatic lipase (EC 3.1.1.3) was immobilized on chitin and chitosan by adsorption and subsequent crosslinking with glutaraldehyde, which was added before (conjugation) or after (crosslinking) washing unbound proteins. Conjugation proved to be the better method for both supports. The properties of free and immobilized enzymes were also investigated and compared. The results showed that the pH optimum was shifted from 8.5 to 9.0 for both the immobilized enzymes. Also, the optimum temperature was shifted from 30 to 40 degrees C for chitin-enzyme and to 45 degrees C for chitosan-enzyme conjugates. The immobilization efficiency is low, but the immobilized enzymes have good reusability and stability (storage and operational). Besides these properties, the immobilized lipases were also suitable for catalyzing esterification reactions of fatty acids and fatty alcohols, both with a medium chain length. According to our results, esterification activities of immobilized lipases were two- and four-fold higher for chitosan- and chitin-enzyme, than for the free enzyme, respectively. The immobilization procedure shows a great potential for commercial applications of the immobilized lipase, a relatively low cost commer...Continue Reading

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Citations

Sep 19, 2012·Applied Microbiology and Biotechnology·Christian DetzelJoachim Jose
Nov 2, 2014·Applied Biochemistry and Biotechnology·Ilyas DeveciBedrettin Mercimek
Nov 2, 2016·Preparative Biochemistry & Biotechnology·Maria Carolina C CorradiniAdriano A Mendes
Apr 24, 2007·Advances in Applied Microbiology·Moh'd Salameh, Juergen Wiegel
Sep 23, 2016·International Journal of Molecular Sciences·Jakub ZdartaTeofil Jesionowski
Sep 5, 2006·Journal of Ethnopharmacology·Katrin SchützAndreas Schieber
Apr 1, 2017·Applied Microbiology and Biotechnology·Faez Iqbal KhanMd Imtaiyaz Hassan
Mar 6, 2021·International Journal of Biological Macromolecules·F Rafiee, M Rezaee

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