Immobilization of primary astrocytes and neurons for online monitoring of biochemical processes by NMR

Developmental Neuroscience
P M AlvesU Sonnewald

Abstract

Immobilization of primary astrocytes and cerebellar granule neurons in basement membrane gel threads and on porous microcarriers, respectively, is described. Five different porous microcarriers were examined for their capabilities to support maturation of neurons, as evaluated by determinations of protein content and glucose uptake. These systems proved suitable for perfusion in the NMR tube for long periods of time. The energetic status of the cells was monitored by 31P-NMR, and a very good temporal resolution (less than 30 min) was achieved with superfused astrocytes; thus, this provides a valuable method for real time NMR investigation of biochemical processes in primary astrocytes. Significantly longer acquisition times (approximately 8 h) were required in order to obtain a spectrum of neurons with a reasonably good signal to noise ratio, even so, the results presented here and obtained with this difficult system are very encouraging.

Citations

Apr 7, 2006·Applied Microbiology and Biotechnology·A S CoroadinhaM J T Carrondo
Jun 22, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Anthony MancusoStephen Pickup
Dec 17, 2004·Journal of Neuroscience Research·Sónia Sá SantosPaula M Alves
Feb 7, 2006·Neurochemistry International·Lorraine BrennanGethin J McBean
Apr 18, 2008·Neurochemistry International·Ying Jin, Lorraine Brennan

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