Immobilization of saccharides and peptides on 96-well microtiter plates coated with methyl vinyl ether-maleic anhydride copolymer

Analytical Biochemistry
A SatohI Matsumoto

Abstract

We have previously reported a method to immobilize protein ligands on microtiter plates coated with methyl vinyl ether-maleic anhydride copolymer (MMAC) [Isosaki, K., et al. (1992) J. Chromatogr. 597, 123-128]. In this study, we improved the MMAC method to efficiently immobilize not only small ligands such as peptides and oligosaccharides, which could not be efficiently immobilized previously, but also heparin via its reducing end. Amino and hydrazino groups were introduced to MMAC-coated microtiter plate wells by coupling to acid anhydride groups of MMAC with 1,6-hexamethylenediamine and adipic acid dihydrazide, respectively. The amino groups introduced were allowed to react with peptides by use of divalent cross-linkers. Hydrazino groups were allowed to react with formyl groups of saccharides by reductive amination. Peptides and oligosaccharides were immobilized in a dose-dependent manner by these methods. In the case of the angiotensin peptide thus immobilized, the detection limit by monoclonal antibodies was as low as 0.1-1 fmol peptide per well. Application of 20-200 nmol oligosaccharides to the well was sufficient to immobilize and subsequently detect lectins. Furthermore, heparin immobilized on the hydrazinocoated wells ...Continue Reading

References

Jun 9, 1995·Journal of Immunological Methods·A NiveleauF Troalen

Citations

Sep 5, 2003·Journal of Immunoassay & Immunochemistry·Tulsidas G Shrivastav
Feb 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Daisuke SugaharaTatsuro Irimura
Aug 4, 2015·Biomaterials·Juliane Salbach-HirschLorenz C Hofbauer
Jun 25, 2002·Chemistry & Biology·Marian C BryanChi-Huey Wong
Apr 2, 2008·Expert Review of Vaccines·Oyindasola Oyelaran, Jeffrey C Gildersleeve
Jul 5, 2017·Chemical Communications : Chem Comm·Gloria Jiménez-SánchezThomas Trimaille
May 22, 2008·Molecular BioSystems·Emiliano GemmaAlison N Hulme
May 3, 2005·Glycoconjugate Journal·Nadezhda V ShilovaNicolai V Bovin
May 6, 2019·Glycoconjugate Journal·Danique J HofToin H van Kuppevelt

Related Concepts

1,6-diaminohexane monohydrochloride
Adipic dihydrazide
methoxyethylene-maleic anhydride copolymer
Adipates
Diamines
Enzyme-Linked Immunosorbent Assay
Liquaemin
Maleates
Oligopeptides
Oligosaccharides

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