Immobilization of the recombinant invertase INVB from Zymomonas mobilis on Nylon-6.

Journal of Industrial Microbiology & Biotechnology
Vanessa Vallejo-BecerraMaría Eugenia Hidalgo-Lara

Abstract

The recombinant invertase INVB (re-INVB) from Zymomonas mobilis was immobilized on microbeads of Nylon-6, by means of covalent bonding. The enzyme was strongly and successfully bound to the support. The activity of the free and immobilized enzyme was determined, using 10% (w/v) sucrose, at a temperature ranging between 15 and 60 degrees C and a pH ranging between 3.5 and 7. The optimal pH and temperature for the immobilized enzyme were 5.5 and 25 degrees C, respectively. Immobilization of re-INVB on Nylon-6 showed no significant change in the optimal pH, but a difference in the optimal temperature was evident, as that for the free enzyme was shown to be 40 degrees C. The values for kinetic parameters were determined as: 984 and 98 mM for Kappm of immobilized and free re-INVB, respectively. Kappcat values for immobilized and free enzymes were 6.1x10(2) and 1.2x10(4) s(-1), respectively, and immobilized re-INVB showed Vappmax of 158.73 micromol h min(-1) mg(-1). Immobilization of re-INVB on Nylon-6 enhanced the thermostability of the enzyme by 50% at 30 degrees C and 70% at 40 degrees C, when compared to the free enzyme. The immobilization system reported here may have future biotechnological applications, owing to the simplicity...Continue Reading

References

Mar 1, 1991·Enzyme and Microbial Technology·J MansfeldH Dautzenberg
Dec 1, 1974·The Biochemical Journal·L GoldsteinM Sokolovsky
Feb 13, 2001·Enzyme and Microbial Technology·F H. IsgroveA T. Andrews
Aug 17, 2006·Journal of Biomaterials Science. Polymer Edition·Hidayet MaziGunay Kibarer
Feb 2, 2008·Journal of Agricultural and Food Chemistry·Vanessa Vallejo-BecerraPedro Antonio García-Ruiz
Feb 1, 1983·Biotechnology and Bioengineering·K ImaiA Fujishima
Apr 1, 1984·Biotechnology and Bioengineering·P Monsan, D Combes

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Citations

Jun 20, 2014·Microbial Cell Factories·Duniesky MartínezLázaro Hernández
Aug 21, 2008·Journal of Industrial Microbiology & Biotechnology·María de Los Angeles Calixto-RomoMaría Eugenia Hidalgo-Lara
Nov 18, 2009·Biotechnology Progress·Pattamawadee Tananchai, Yusuf Chisti

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