Immobilized and Free Cells of Geotrichum candidum for Asymmetric Reduction of Ketones: Stability and Recyclability

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Hui LiuBi-Shuang Chen

Abstract

Marine-derived fungus Geotrichum candidum AS 2.361 was previously reported by our group as an active strain for the enantioselective reduction of ketones. Although some other Geotrichum strains were also found from the terrestrial sources, information on their stability and reusability is scarce. Herein, the stabilities-in terms of pH tolerance, thermostability, and storage stability, and reusability-of G. candidum AS 2.361 were described for the asymmetric reduction of a series of aromatic ketones. Two differently immobilized cells (agar immobilization and calcium alginate immobilization) as well as free cells were prepared. For three substrates (1-(3-bromophenyl) ethan-1-one (1b), 1-(2-chlorophenyl) ethan-1-one (1d), and acetophenone (1g)) immobilized cells on agar showed a great improvement in the bioreduction activities compared to the free cells, increasing yields up to 97% with ee values of 99%. Cells immobilized on agar/calcium alginate could maintain more than 90% of the original activities within the assayed pH ranges of 3.5⁻11, while free cells were highly sensitive to alkaline and acidic conditions. Concerning thermostability, immobilized cells on agar kept 99% of their original activities after incubation at 60 °C f...Continue Reading

References

Mar 16, 2007·Microbial Cell Factories·Pernilla TurnerEva Nordberg Karlsson
May 16, 2009·Biotechnology Advances·J D CarballeiraJ V Sinisterra
Jun 11, 2009·Angewandte Chemie·Christian Hertweck
Aug 20, 2009·Applied Biochemistry and Biotechnology·Mário A P NunesMaria H L Ribeiro
Nov 27, 2009·Marine Biotechnology·Lenilson Coutinho RochaAndré Luiz Meleiro Porto
May 19, 2010·Marine Drugs·Sreyashi SarkarJoydeep Mukherjee
Jul 7, 2011·Marine Drugs·Antonio Trincone
Dec 14, 2011·Marine Biotechnology·Lenilson C RochaAndré L M Porto
Mar 12, 2013·Chemical Society Reviews·Manfred SchreweAndreas Schmid
Aug 15, 2015·Marine Biotechnology·Lenilson C RochaAndré L M Porto
Jun 1, 2016·Enzyme and Microbial Technology·María VillalbaCristina Otero
Jan 12, 2017·Applied Microbiology and Biotechnology·Yu-Guo ZhengZhi-Qiang Liu
Dec 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana D Q MeloFrancisco A Dias Filho
Dec 22, 2017·Applied Microbiology and Biotechnology·Hui LiuBi-Shuang Chen

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Methods Mentioned

BETA
scanning electron microscopy
NMR

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