Immobilized enzymes as analytical reagents

Applied Biochemistry and Biotechnology
G G Guilbault


Immobilized enzymes are becoming increasingly popular as analytical reagents because of their reusability, stability, and sensitivity to many inhibitors that would seriously interfere in assays using soluble enzymes. In this article, some of the kinetic and catalytic effects of immobilized enzymes in analysis will be discussed. The shift of the activity-pH profile curves on immobilization, the changes in temperature dependence. the inhibitor constants (K1). Michaelis constants (K m ), and the maximum velocity (Vmax). plus others, will be discussed. Finally, the use of these immobilized enzymes in fluorometric and electrochemical monitoring systems will be shown, and the future of these reagents in various areas will be discussed. A survey of enzyme electrodes will be presented as an example of the use of immobilized enzymes. Application of immobilized enzyme technology to the assay of BUN, glucose, uric acid, amino acids, ethanol. and other metabolites will be discussed.


Aug 9, 1976·Biochemical and Biophysical Research Communications·R K Kobos, G A Rechnitz
Jan 17, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Kumar, G D Christian
Mar 1, 1978·Die Naturwissenschaften·M von Ardenne, P G Reitnauer
Dec 1, 1978·The Journal of Pharmacy and Pharmacology·C J OlliffJ M Wright
Dec 1, 1977·Analytica Chimica Acta·G A RechnitzC R Gebauer
Mar 29, 1979·Nature·G A RechnitzM E Meyerhoff
Jan 27, 1978·Science·G A RechnitzM E Meyerhoff
Sep 1, 1978·Analytical Chemistry·W C White, G G Guilbault
Feb 1, 1977·Analytica Chimica Acta·C E Hjemdahl-MonsenG A Rechnitz
May 1, 1977·Analytical Chemistry·M Mascini, G G Guilbault
May 1, 1977·Analytica Chimica Acta·C P HsiungG G Guilbault
Jun 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y KamenoS Baba
Jul 1, 1977·Biotechnology and Bioengineering·I SatohS Suzuki
Oct 1, 1977·Analytical Chemistry·D N GrayB Watson
Jul 1, 1976·Archives of Biochemistry and Biophysics·R K Kobos, G A Rechnitz
Jul 1, 1976·Analytica Chimica Acta·T Cserfalvi, G G Guilbault
Aug 1, 1976·Analytica Chimica Acta·G JohanssonL Ogren
Jul 1, 1975·Analytical Chemistry·C Tran-Minh, G Broun
Feb 1, 1976·Biotechnology and Bioengineering·I SatohS Suzuki
May 1, 1976·Analytical Chemistry·D S Papastathopoulos, G A Rechnitz
Jun 1, 1976·Analytica Chimica Acta·G Johansson, L Ogren
Nov 1, 1972·Acta diabetologica latina·S Raptis, E F Pfeiffer
Dec 29, 1974·Biochimica Et Biophysica Acta·P Davies, K Mosbach
Sep 1, 1971·Analytica Chimica Acta·G G Guilbault, E Hrabankova
Oct 1, 1974·Analytical Chemistry·M Nanjo, G G Guilbault
Aug 1, 1974·Biotechnology and Bioengineering·P R CouletD C Gautheron
Jun 1, 1972·Analytica Chimica Acta·G G Guilbault, G J Lubrano
May 1, 1973·Analytica Chimica Acta·G G Guilbault, G J Lubrano
Sep 14, 1973·Biochimica Et Biophysica Acta·H NilssonK Mosbach
Oct 1, 1973·Analytica Chimica Acta·G NagyG G Guilbault
Apr 1, 1973·Analytical Chemistry·G J PaparielloC M Shearer
Feb 1, 1974·Analytical Chemistry·H Thompson, G A Rechnitz
Jul 1, 1972·Analytical Chemistry·R Llenado, G A Rechnitz
Oct 1, 1971·Analytica Chimica Acta·G G Guilbault, F R Shu
Sep 1, 1971·Analytical Chemistry·R Llenado, G A Rechnitz
Feb 1, 1971·Analytical Chemistry·G A Rechnitz, R Llenado
Jan 1, 1970·Analytical Chemistry·D L WilliamsA Korosi
Feb 1, 1970·European Journal of Biochemistry·J M Varga, H Y Neujahr
Dec 1, 1970·Analytical Chemistry·G G Guilbault, E Hrabankova
Apr 9, 1969·Journal of the American Chemical Society·G G Guilbault, J G Montalvo
Oct 1, 1965·Analytical Chemistry·E K BaumanD N Kramer
Oct 13, 1967·Science·S J Updike, G P Hicks
Jun 3, 1967·Nature·S J Updike, G P Hicks
Jun 1, 1981·Analytical Chemistry·I KaruheT Kada
Feb 1, 1981·Analytical Chemistry·C L YuanG G Guilbault
Dec 13, 1978·Journal of Clinical Engineering·C E McCullough, L S Baker
Oct 31, 1962·Annals of the New York Academy of Sciences·L C CLARK, C LYONS


Apr 1, 1987·Applied Biochemistry and Biotechnology·L G Reddy, V Shankar
Aug 10, 2006·Applied Microbiology and Biotechnology·Asha ChaubeyGhulam N Qazi
Oct 3, 2008·Applied Biochemistry and Biotechnology·Lili Gai, Daocheng Wu
Apr 1, 1993·Journal of Biotechnology·S Gite, V Shankar
Aug 1, 1985·Analytical Chemistry·S D CarasK Schmitt
Jan 1, 1993·Critical Reviews in Biotechnology·L G Reddy, V Shankar
Mar 23, 2017·Biochemistry. Biokhimii︠a︡·L M Kulishova, D O Zharkov

Related Concepts

Immunoglobulin Km Allotypes
Uric Acid
Blood Urea Nitrogen

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.