PMID: 2779Feb 1, 1976

Immobilized glucuronosyltransferase for the synthesis of conjugates

Journal of Medicinal Chemistry
I ParikhC Fenselau


Partially purified rabbit liver UDPglucuronosyltransferase is immobilized on agarose by the cyanogen bromide activation method. Both soluble and matrix-bound enzyme preparations display very similar Km and pH optimum. The storage stability of the immobilized enzyme at 4 degrees is 5-10 times improved over the soluble preparations. The agarose-bound UDPglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70%. The matrix-bound enzyme is reusable over an extended period of time and offers an easy and convenient synthetic tool for various drug glucuronides.


Sep 1, 1977·Biotechnology and Bioengineering·A KlibanovI V Berezin
May 20, 1998·Natural Product Reports·A V Stachulski, G N Jenkins
Aug 1, 1995·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H HeK K Midha
Mar 1, 1991·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C J JacksonK K Midha
May 1, 1979·Artificial Organs·S S SoferB J Van Wie

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