Immobilized metal-ion affinity chromatography: imidazole proton pump and chromatographic sequelae. II. Chromatographic sequelae

Journal of Molecular Recognition : JMR
E Sulkowski

Abstract

Isocratic elution with imidazole of some protein models from a chelating gel, CSFF-IDA-M(II), resulted in their desorption owing to the low pH(6-->4) of the mobile, phase rather than to the imidazole itself (imidazole-generated fall in pH; proton pump). Gradient elution with imidazole was best accomplished when the chelating gel was initially converted into its imidazole complex, CSFF-IDA-M(II)Im. The exploitation of the imidazole-generated proton pump in the IMAC of proteins may enhance the versatility of this type of chromatography.

References

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Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·V Gaberc-Porekar, V Menart
Jul 12, 2003·Journal of Biomolecular Structure & Dynamics·Hsuan-Liang LiuChia-Ming Hsu
May 23, 2008·Journal of Chromatography. a·Frantz Le DévédecMircea Alexandru Mateescu

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